The Effects of Steroids and Nonsteroidal Antiinflammatory Agents on Proliferation of Human Ocular Fibroblast.

Lee, Jong Soo; Jung, Wook; Choi, Young Rak; Kim, Yoo Seon

Original Article

Journal of the Korean Ophthalmological Society 1999;40(6):1496-1502.
Published online June 1, 1999.

The Effects of Steroids and Nonsteroidal Antiinflammatory Agents on Proliferation of Human Ocular Fibroblast.

Jong Soo Lee, Wook Jung, Young Rak Choi, Yoo Seon Kim

Department of Ophthalmology, The Pusan National University Medical College.

스테로이드성과 비스테로이드성 항염증제가 배양된 인체 익상편 섬유아세포의 증식억제에 미치는 영향

이종수(Jong Soo Lee),정욱(Wook Jung),최영락(Young Rak Choi),김유선(Yoo Seon Kim)

Abstract

Corticosteroid, 5-fluorouracil and Mitomycin C(MMC) have been used to suppress proliferation of subconjunctival connective tissue. However, the various complication and undesirable side effects associated with the use of these drugs have prompted the search for alternative drugs with less toxicity. In present study, I have evaluated the effect of corticosteroid(dexamethsone sodium phophate), cyclooxygenase inhibitor(diclofenac sodium) and lipoxygenase inhibitor(nordihydroguaratic acid, NDGA) to inhibit human pterygial fibroblast proliferation in tissue culture. Human pterygial fibroblast were cultured and exposed to various concentration of each drug for 1, 2 and 3 days. Cell viability was measured using MTT assay. There was no different in antiproliferative activity between nonsteroidal antiinflammatory agents(NSAIDs) and steroid. Dexamethasone caused inhibition of proliferation with LD 50 of 0.5mM by day 2, NDGA of 0.5mM by day 3 and diclofenac sodium of 1.0mM by day 3. These results suggest that NSAIDs may be useful as both antiproliferative and less toxicity agents.

Key Words: Dexamethasone;Fibroblast;NSAIDs