Effect of Mitomycin C and 5-Fluorouracil on Cultured Human Nasal Mucosa Fibroblasts.

Kim, Keun Hae; Kim, In Taek

doi:2011.52.2.233

J Korean Ophthalmol Soc > Volume 52(2); 2011 > Article

Original Article

Journal of the Korean Ophthalmological Society 2011;52(2):233-240.
DOI: https://doi.org/10.3341/jkos.2011.52.2.233 Published online February 15, 2011.

Effect of Mitomycin C and 5-Fluorouracil on Cultured Human Nasal Mucosa Fibroblasts.

Keun Hae Kim, In Taek Kim

¹Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. kimkh@cu.ac.kr
²Department of Ophthalmology, Kyungpook National University School of Medicine, Daegu, Korea.

배양된 사람 코점막 섬유아세포에 대한 마이토마이신 C와 5-플루오로우라실의 효과

김근해¹⋅김인택²

Department of Ophthalmology, Catholic University of Daegu School of Medicine¹, Daegu, Korea Department of Ophthalmology, Kyungpook National University School of Medicine², Daegu, Korea

Abstract

PURPOSE
To investigate the effects of two antimetabolites, mitomycin C (MMC) and 5-fluorouracil (5-FU), on proliferation of cultured human nasal mucosa fibroblasts. METHODS: Human nasal mucosa fibroblasts were primarily cultured, and exposed to various concentrations of MMC and 5-FU for 5 minutes. Control fibroblasts were exposed to only DMEM media without the drugs. Effect of drugs on cell morphology was observed by phase-contrast microscopy. Cell viability and apoptosis were measured using MTT [3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-tetrazolium bromide] assay and Acridine orange/Hoechst (AO/HO) staining, respectively. RESULTS: In both experimental groups exposed to MMC and 5-FU, fibroblasts maintained standard spindle shape. The MTT assay showed that both MMC and 5-FU inhibited fibroblast proliferation in a dose dependent manner. AO/HO staining showed apoptotic cells in both experimental groups. CONCLUSIONS: Both MMC and 5-FU have an antiproliferative effect on fibroblasts in vitro at least through induction of apoptosis. Therefore, adjuvant use of either MMC or 5-FU during endonasal dacryocystorhinostomy may improve the clinical outcome by inhibiting proliferation of the nasal mucosa.

Key Words: 5-fluorouracil;Mitomycin C;Nasal mucosa fibroblast