Effects of Prednisolone and Loteprednol Eyedrops on the Proliferation of Human Tenon's Capsule Fibroblasts.

Kang, Sun Hee; Kim, Jae Woo

doi:2013.54.9.1423

J Korean Ophthalmol Soc > Volume 54(9); 2013 > Article

Original Article

Journal of the Korean Ophthalmological Society 2013;54(9):1423-1428.
DOI: https://doi.org/10.3341/jkos.2013.54.9.1423 Published online September 15, 2013.

Effects of Prednisolone and Loteprednol Eyedrops on the Proliferation of Human Tenon's Capsule Fibroblasts.

Sun Hee Kang, Jae Woo Kim

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr

로테프레드놀과 프레드니솔론 점안제가 테논낭 섬유아세포의 증식에 미치는 영향

강선희⋅김재우

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea

Abstract

PURPOSE
To compare the effect of loteprednol etabonate (LE) with prednisolone acetate (PDA) drops on the proliferation of human Tenon's capsule fibroblasts (HTFBs). METHODS: Primarily cultured HTFBs were treated with serially diluted PDA and LE for 3 days. Cellular survival was determined by a rapid colorimetric assay using MTT. RT-PCR was performed to determine the relative expression of TGF-beta mRNA in response to LE and PDA. RESULTS: PDA inhibited proliferation of HTCF in a dose-dependent manner and LE inhibited significantly the proliferation of HTCF at the higher concentration of 50 microg/ml (p < 0.05). Compared to LE, PDA inhibited proliferation of HTCF significantly at each diluted concentration (p < 0.05). Expressions of TGF-beta were decreased as the concentration of both PDA and LE increased. PDA decreased expression of TGF-beta more significantly compared to LE at each concentration (p < 0.05). CONCLUSIONS: Although LE has offered promising anti-inflammatory efficacy with decreased impact on intraocular pressure, LE may be less effective than PDA in inhibiting fibroblast proliferation and may be not comparable to PDA in preventing excessive scarring after glaucoma filtering surgery.

Key Words: Fibroblasts;Loteprednol etabonate;Prednisolone acetate;Proliferation;TGF-beta