| The Effect of Anti-inflammatory Agents on the Permeability of Trabecular Meshwork Cell Monolayers. |
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Hye Ri Kang, Jung Hoon Lee, Jae Woo Kim |
1Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
2Catholic Union Eye Clinic, Daegu, Korea. |
| 소염제가 섬유주단층세포층의 투과성에 미치는 효과 |
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강혜리1⋅이정훈2⋅김재우1 |
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대구가톨릭대학교 의과대학 안과학교실1, 가톨릭연합안과의원2 |
Correspondence:
Jae Woo Kim,
Email: jwkim@cu.ac.kr
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Received: 13 October 2016 • Revised: 5 January 2017 • Accepted: 18 September 2017 |
| Abstract |
PURPOSE
To compare the effects of anti-inflammatory agents, specifically bromfenac, loteprednol, and prednisolone, on the permeability of cultured human trabecular meshwork cell (HTMC) monolayers. METHODS: HTMCs were cultured until confluency in the inner chamber of Transwell, then exposed to 1/1,000 or 1/500 diluted commercial 0.1% bromfenac, 0.5% loteprednol, and 1% prednisolone for 24 hours. The permeabilities of carboxyfluorescein through the HTMC monolayer were measured with a spectrofluorometer after 2 hours in the outer chamber. Cellular viabilities were assessed with an 3-[4,5–dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. RESULTS: Bromfenac and loteprednol diluted at 1/1,000 or 1/500 did not significantly affect the cellular survival (p > 0.05). Bromfenac did not affect the permeability via the HTMC monolayer (p > 0.05) and loteprednol decreased the permeability (p < 0.05). In addition, 1/2,000 prednisolone also decreased the permeability (p < 0.05). CONCLUSIONS: Among the anti-inflammatory agents, the non-steroidal anti-inflammatory agent bromfenac did not affect the permeability, while loteprednol and prednisolone decreased the permeability through the HTMC monolayer. Thus, loteprednol and prednisolone may decrease the trabecular outflow. |
| Key Words:
Bromfenac;Loteprednol;Permeability;Prednisolone;Trabecular meshwork cells |
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