| Effects of Pupil Dilation and Constriction Agents on Trabecular Meshwork Cells. |
|
Jeong Hun Lee, Jae Woo Kim |
|
Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 동공 산동제와 축동제가 섬유주세포에 미치는 영향 |
|
이정훈⋅김재우 |
|
Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea |
|
|
| Abstract |
PURPOSE
To investigate the effects of pupil dilation and constriction agents on the survival and production of nitric oxide (NO) in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 0, 0.01, and 0.1 mg/ml of tropicamide, cyclopentolate, atropine, or pilocarpine for 2 hours. Cellular survival and production of NO were assessed using the MTT assay and Griess assay, respectively. RESULTS: Tropicamide, cyclopentolate, atropine, and pilocarpine decreased cellular survival at the concentration of 0.1 mg. At the concentration of 0.01 mg/ml, all agents decreased production of NO to some extent, although the reduction was not statistically significant. CONCLUSIONS: Pupil dilation and constriction agents may be toxic to HTMC if used at high concentrations or if used frequently in the short-term but may not affect trabecular outflow. |
| Key Words:
Atropine;Cyclopentolate;Pilocarpine;Trabecular meshwork cells;Tropicamide |
|
PDF Links
Full text via DOI
Download Citation
