| Effect of Topically Applied Human Epidermal Growth Factor on Stromal Keratocyte Activity in Experimental Corneal Alkali Wounds. |
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Moon Jung Kim, Roo Min Jun, Jang Hyun Chung |
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Department of Ophthalmology, College of Medicine, Ewha Medical Research Center, Ewha Womans University, Seoul, Korea. |
| 가토 각막 알칼리 화상에서 인간 표피성장인자의 국소 점안이 각막실질세포의 활성화에 미치는 영향 |
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김문정(Moon Jung Kim),전루민(Roo Min Jun),정장현(Jang Hyun Chung) |
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| Abstract |
PURPOSE
The Influence of topically applied human epidermal growth factor(hEGF) on stromal keratocyte was evaluated in the experimental corneal alkali wounds. METHODS: Corneal alkali wounds were induced by applying a 5.5 mm round filter paper soaked in 1N NaOH onto the center of rabbit cornea for 60 seconds. The eyes were treated topically with 5 micrometer/ml hEGF(DaeWoong Pharma. Co. Ltd. Seoul, Korea) or PBS, 3 times per day. After 3- and 28-days treatment, the corneas were processed for transmission electron microscopy(TEM). RESULTS: After 3-days treatment, the basement membrane integrity was maintained and the stromal keratocyte remained damaged in both groups. However, after 28-days treatment, the basement membrane was disrupted and the keratocytes were activated in both groups. CONCLUSIONS: The findings of present study indicated that the optimal concentration of hEGF which is known to stimulate the epithelial healing was failed to affect the keratocyte activities as long as the basement membrane integrity was maintained. |
| Key Words:
Corneal alkali wounds;hEGF;Keratocyte |
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