| Optimal Concentration of hEGF for Epithelial Healing in Experimental Corneal Alkali Wounds. |
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Roo Min Jun, Jang Hyun Chung |
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Department of Ophthalmology, College of Medicine, Ewha Womans University, Seoul, Korea. junrumin@yahoo.co.kr |
| 각막 알칼리 화상에서 각막 상피의 치유를 촉진시키는 인간 표피성장인자의 적정 농도 |
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전루민(Roo Min Jun),정장현(Jang Hyun Chung) |
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| Abstract |
PURPOSE
An optimal concentration of hEGF to enhance epithelial healing was determined in the rabbit eyes after alkali wounds. METHODS: Alkali-injured corneas (=5.5 mm, 1N NaOH, 60s) were treated with 0 (control), 2, 5, 10, and 50microgram/ml hEGF 3 times per day. The measurement of epithelial healing rate, transmission electron microscopy and immunohistochemical observation were performed after 7 days treatment. RESULTS: No significant difference was observed in the rates of epithelial healing between control and each hEGF-treated group. Among the tested concentrations, 5microgram/ml hEGF induced the most active proliferation of basal cells and 50microgram/ml hEGF remarkably produced a vascular ingrowth to the central wound area. The number of epithelial layers was increased by hEGF in a concentration-dependent manner. CONCLUSIONS: A low concentration of hEGF may selectively enhance epithelial healing without inducing corneal neovascularization. The optimal concentration of hEGF for the stimulation of basal cell proliferation appears to be 5 microgram/ml in rabbit corneal alkali wounds. |
| Key Words:
Corneal alkali wound;Epithelial healing;hEGF |
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