Proliferation of Corneal Endothelial Cells by Delivery of SV40 Large T Antigen.

Cho, Kyung Sun; Song, In Kyung; Fleming, Timothy P; Joo, Choun Ki

Original Article

Journal of the Korean Ophthalmological Society 2001;42(9):1331-1335.
Published online September 1, 2001.

Proliferation of Corneal Endothelial Cells by Delivery of SV40 Large T Antigen.

Kyung Sun Cho, In Kyung Song, Timothy P Fleming, Choun Ki Joo

¹Department of Ophthalmology and Visual Science, Kangnam St. Mary's Hospital.
²Catholic University Medical College, Research Institutes of Medical Science, Seoul, Korea.
³Department of Ophthalmology and Visual Science, Washington University School of Medicine.

SV40 large T antigen 을 이용한 각막내피세포의 증식유도

조경선(Kyung Sun Cho) , 송인경(In Kyung Song) , 주천기(Choun Ki Joo) , (Timothy P. Fleming)

Abstract

PURPOSE
To determine whether the delivery of the SV40 large T-antigen is a feasible method for transiently inducing proliferation of corneal endothelial cells, we delivered liposome-protein complex into bovine corneal endothelial cells(BCEC). METHOD: SV40 large T-antigen protein was introduced into BCEC and positive cells were identified by immunohistochemistry. Quiescent BCECs were double-labeled using BrdU as a measure of de novo DNA synthesis and the Ki-67 was detected by standard immunohistochemical methods. RESULT: The treatment of quiescent BCECs with large T antigen caused an increase in BrdU incorporation and Ki-67 expression. It was tested by time-course study. CONCLUSION: This finding suggests that liposome-mediated delivery of transforming proteins could be a method to transiently induce corneal endothelial cell proliferation.

Key Words: BrdU;Cell proliferation;Ki-67;Protein delivery;SV40 large T antigen