| Effect of Dipyridamole on the Reactive Oxygen Species and Oxidative Stress in Trabecular Meshwork Cells. |
|
Keun Woo Lee, Jae Woo Kim |
|
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 섬유주세포에서 Dipyridamole이 활성산소종과 산화스트레스에 미치는 영향 |
|
이근우⋅김재우 |
|
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea |
|
|
| Abstract |
PURPOSE
To investigate the effects of dipyridamole (DPD) on the production of reactive oxygen species (ROS) and oxidative stress in cultured human trabecular meshwork cells (HTMC). METHODS: Antioxidant activity of DPD was determined by DPPH assay. Primarily cultured HTMC were exposed to 0, 20, and 50 microm DPD using serum-deprived media. The effect of DPD on the production of ROS was assessed with the DCHFDA assay. The effect of DPD on the t-butyl hydroperoxide (tBHP)-induced oxidative stress was assessed with resazurin assay. RESULTS: DPD showed significant antioxidant activity. DPD significantly decreased the production of ROS (p < 0.05) and improved cellular activity significantly after treatment with t-BHP (p < 0.05). DPD did not affect the generation of nitric oxides. CONCLUSIONS: DPD suppressed the formation of ROS and possessed cytoprotective activity against the oxidative stress in HTMC. |
| Key Words:
Antioxidant;Dipyridamole;Reactive oxygen species;Trabecular meshwork cells |
PDF Links
Full text via DOI
Download Citation
