| Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells. |
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Seung Hee Lee, Jae Woo Kim |
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Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 섬유주세포에서 Erythropoietin이 일산화질소의 생성에 미치는 영향 |
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이승희⋅김재우 |
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Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea |
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| Abstract |
PURPOSE
To investigate the effects of erythropoietin (EPO) on the production of nitric oxide in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 0, 0.5, and 1.0 U/ml EPO using serum-deprived media for 2 days. Production of nitric oxide and cellular survival were assessed with Griess assay and MTT assay, respectively. Expression of EPO mRNA receptor and activity of eNOS were assessed with RT-PCR. RESULTS: EPO did not affect the survival of HTMC (p > 0.05). EPO increased the production of nitric oxide (p < 0.05) accompanied with increased eNOS activity. CONCLUSIONS: EPO had no effect on the cellular survival under serum-deprived conditions. EPO increases the production of nitric oxide by increasing eNOS activity. |
| Key Words:
Erythropoietin;Nitric oxide;Trabecular meshwork cells |
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