| The Role of Nuclear Factor Kappa B on Lens Epithelial Cells after Ultraviolet B Irradiation. |
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Do Hyung Lee, Jung Kweon Kim, Sae Hoon Oh, Eung Kwon Kim, Choun Ki Joo |
1Department of Ophthalmology, Ilsan Paik Hospital, College of Medicine, Inje University, Korea.
2The Institute of Vision Research, Department of Ophthalmology, Yonsei University, College of Medicine, Korea.
3Department of Ophthalmology, Kangnam St. Mary's Hospital, The Catholic University of Korea, College of Medicine, Korea. ckjoo@cmc.cuk.ac.kr |
| 자외선 조사를 한 수정체 상피세포에서 Nuclear Factor Kappa B의 활성화에 관한 연구 |
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이도형 ( Lee Do Hyeong ) , 김정권 ( Kim Jeong Gwon ) , 오세훈 ( O Se Hun ) , 김응권 ( Kim Eung Gwon ) , 주천기 ( Ju Cheon Gi ) |
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| Abstract |
PURPOSE
This study was performed to determine the role of nuclear factor kappa B (NF-kappa B) on the lens epithelial cell death after ultraviolet (UV) irradiation. METHODS: Simian virus 40 transfected human lens epithelial cells (HLE B-3 cells) were used in this study. UVB located at 10cm from the bottom was irradiated during 1, 2, 3 and 4 minutes. To measure the cytotoxicity MTT assay was used. Translocation of NF-kappa B was examined by immunocytochemistry with anti NF-kappa B p65 antibody and electrophoretic mobility shift assay (EMSA). To confirm the role of NF-kappa B, the cells were pretreated with sulfasalazine, a specific inhibitor of NF-kappa B, for 30 minutes before irradiation, and cytotoxicity and translocation of NF-kappa B were evaluated. RESULTS: UV irradiation produced a progressive cytotoxic effect in cultured HLE B-3 cells after 1 minute and maximum cytotoxicity was reached after 3 minutes irradiation. When HLE B-3 cells were irradiated with UVB, the translocation of NF-kappa B was observed in immunocytochemistry. These translocations were peaked 6 hours after UV irradiation in EMSA. In HLE B-3 cells pretreated with sulfasalazine, the translocation of NF-kappa B was blocked. The cellular death after UV irradiation was markedly blocked by sulfasalazine. UV irradiation can translocate NF-kappa B and sulfasalazine is a useful blocking agent in this pathway. In addition, sulfasalazine can prevent cellular death after UV irradiation. CONCLUSIONS: These findings suggest that NF-kappa B plays an important role in cellular death after UV irradiation. |
| Key Words:
Human lens epithelial cells;Nuclear factor kappa B;Ultraviolet irradiation |
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