The Effect of the Remnants after Cataract Extraction on the Lens Epithelial Cell Culture.

Do, Song Jun; Kang, Sug Jae; Lee, Jae Hwan; Oh, Jung Hyub; Lee, Jun Hee

Original Article

Journal of the Korean Ophthalmological Society 2002;43(5):872-882.
Published online May 1, 2002.

The Effect of the Remnants after Cataract Extraction on the Lens Epithelial Cell Culture.

Song Jun Do, Sug Jae Kang, Jae Hwan Lee, Jung Hyub Oh, Jun Hee Lee

¹MANUBASE Eye Clinic, Korea.
²Department of Ophthalmology, Medical College Inha University, Inchon, Korea. eye@inha.com
³Department of Emergency Medicine, Medical College Inha University, Inchon, Korea.

백내장수술 후 잔류물질의 수정체상피세포배양에 대한 영향

도송준 ( Song Jun Do ) , 강석재 ( Sug Jae Kang ) , 이재환 ( Jae Hwan Lee ) , 오중협 ( Jung Hyub Oh ) , 이준희 ( Jun Hee Lee )

Abstract

PURPOSE
To assess the effect of the remnants after lens extraction on posterior capsular opacification with lens epithelial cell culture through in vitro capsular bag model. METHODS: After isolating porcine lens capsules, sterile non-toxic PMMA (polymethyl- mathacrylate) tension ring was inserted into the capsule. These were placed in organ culture medium up to 6 weeks. The grade of cell coverage of the posterior lens capsule was recorded to check the proliferative activity. RESULTS: In the process of cell culture, outgrowth of the epithelial cells was observed across the posterior capsule after a lag period. The rate of cell coverage was dependent upon the added factors. The proliferative activity was the greatest in the group where lens cortical and nuclear materials were added, and other groups showed no difference from a control group. CONCLUSIONS: To reduce posterior capsular opacification, it is important that we should not leave the lens cortical material behind during cataract surgery.

Key Words: In vitro capsular bag model;Lens epithelial cell;Posterior capsular opacity