The Changes of the Retinal Ganglional Cells in the Pressure-induced Ischemic Rabbit Retina.

Park, Tae Kwann; Son, Won Sub; Kwak, Hyung Woo

Original Article

Journal of the Korean Ophthalmological Society 2001;42(5):772-782.
Published online May 1, 2001.

The Changes of the Retinal Ganglional Cells in the Pressure-induced Ischemic Rabbit Retina.

Tae Kwann Park, Won Sub Son, Hyung Woo Kwak

Department of Ophthalmology, Kyung Hee University Hospital.

허혈유도 가토망막의 망막신경절세포 변화

손원섭(Won Sub Son),곽형우(Hyung Woo Kwak),박태관(Tae Kwann Park)

Abstract

PURPOSE
There were many studies on the distributions of the retinal ganglion cells(RGC) in the experimental model of the retinal ischemia. RGC was known to be more sensitive to the ischemic injury than the other types of the retinal cells. So, we would identify the changes of the retinal ganglion cell morphologies and distribution after the iatrogenic retinal ischemia induced by intraocular pressure(IOP) elevation. METHODS: Eight pigmented and six white rabbits were used and retinal ischemia was induced by increasing IOP higher than 120 mmHg for 60 minutes. Electroretinogram were recorded at 6 days or 13 days, and histologic findings were observed at 7 or 14 days. RESULTS: After 7 days, RGC densities decreased, cytoplasmic staining disappeared, and the intranuclear hyperpigmentation was noted. RGC densities decreased significantly at 14 days. In the vertical retinal section, some flattening of retinal ganglion cell layer and inner plexiform layer was observed. Changes in the cellular morphologies were prominent. CONCLUSIONS: It may be more appropriate to examine both the retinal whole-mount and the vertical tissue section for the estimatation of the changes of retinal ganglion cell layer in the pressure-induced retinal ischemia.

Key Words: Retinal ganglion cell;Pressure-induced retinal ischemia;Retinal whole-mount