| The Effect on the Retinal Vessels of rhEPO in a Rat Model of Oxygen-Induced Retinopathy. |
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Chang Hyun Park, Tae Kwann Park, Seung Joon Lee, Young Hoon Ohn, Sung Woon Kim |
1Department of Ophthalmology, Soonchunhyang University, College of Medicine, Seoul, Korea. genophilus@hanmail.net
2Department of Pediatrics, Soonchunhyang University, College of Medicine, Seoul, Korea.
3Department of Ophthalmology, Kangwon University, College of Medicine, Gangwon, Korea.
4Department of Internal Medicine, Kyung Hee University, College of Medicine, Seoul, Korea. |
| 산소로 유도된 망막증 모델에서 인간재조합 적혈구생성인자가 망막혈관에 미치는 영향 |
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박창현1,박태관1,김성신2,이승준3,온영훈1,김성운4 |
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Department of Ophthalmology, Soonchunhyang University, College of Medicine1, Seoul, Korea Department of Pediatrics, Soonchunhyang University, College of Medicine2, Seoul, Korea Department of Ophthalmology, Kangwon University, College of Medicine3, Gangwon, Korea Department of Internal Medicine, Kyung Hee University, College of Medicine4, Seoul, Korea |
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Correspondence:
Chang Hyun Park, M.D.1 |
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| Abstract |
PURPOSE
To evaluate the effect of recombinant human erythropoietin (rhEPO) on the retinal vessels in a rat model of oxygen-induced retinopathy (OIR). METHODS: Twenty-six Sprague-Dawley rat siblings were exposed to seven cycles each of hyperoxia (80% O2, 23.5 hours) and room air recovery (0.5 hours), followed by exposure to room air for five days. After each of the test group and control group was divided into two groups of rhEPO injected group and normal saline injected group, 400 u/kg of rhEPO or normal saline were intraperitoneally injected, respectively on the third, fifth, and eighth days of the experiment. Retinal neovascularization and capillary densities were compared between the rats on the eighth and fourteenth day after injection. The extent of VEGF164, and IGF-1 mRNA expression was measured in the rats on the 14th day post-injection. RESULTS: Neovascularization and capillary density was the most increased in the rhEPO injected test group, but the avascular area was widely distributed in the vicinity of the retina. The degree of VEGF164 mRNA expression was highest in the test group that had been injected with normal saline. We found no difference in IGF-1 mRNA levels between the groups. CONCLUSIONS: We can infer that rhEPO can be a potent factor for inducing retinal neovascularization. |
| Key Words:
Erythropoietin;IGF-1;Neovascularization;OIR model;VEGF164 |
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