Journal of the Korean Ophthalmological Society 1999;40(9):2395-2400.
Published online September 1, 1999.
Optimal Concentrations of hEGF for Epithelial and Endothelial Healing in Corneal Alkali Wounds: An Organ Cultured Study.
Moon Jung Kim, Soo Min Paek, Jang Hyun Chung
Department of Ophthalmology, Ewha Medical Researvh Center, College of Medicine, Ewha Womans University, Mokdong Hospital, Seoul, Korea.
각막 알칼리 화상에서 각막 상피 및 내피의 치유를 촉진시키는 인간 표피성장인자의 적정 농도 :각막 배양 연구
김문정(Moon Jung Kim),백수민(Soo Min Paek),정장현(Jang Hyun Chung)
Abstract
Optimal concentrations of hEGF for epithelial and endothelial healing after alkali-injury were determined in an organ cultured system. By using a 5.5 mmround filter paper, corneal alkali wounds (1N NaOH, 60sec.) were induced in the rabbit eyes and then the whole cornea with 1 mmscleral rim were excised.The excised corneas were immersed in the Eagle`s minimum essential medium supplemented with 5% Dextran, 1%antibiotics and different concentrations of hEGF (DaeWoong Pharma.Co.Ltd. Seoul, Korea), i.e., O (control), 0.01, 0.1, 1.0, 10, 100ng/ml. After tryphan blue staining, morphometric analysis was performed at 1, 2, 3, 4 and 6 days time periods. The healing rates were calculated by using an image analysis system. After 7 days, the corneas were processed for an immunohistochemical observation of proliferating cell nuclear antigen (PCNA) positive cells in the re-surfaced epithelium. Epithelial re-surfacing was significantly enhanced by the hEGF treatment at a concentration of 1.0ng/ml. The thickness of regenerated epithelium appeared to be increased in a dose-dependent man-ner. The rate of endothelial healing was increased at the concentration of 100ng/ml. The results indicate that epithelial healing was stimulated at about 100 times lower concentration of hEGF than endothelial healing.
Key Words: Corneal alkali wounds;Epithelial and endothelial healing;hEGF


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