A Study on Measurements of Retinal Blood Flow Using a Fluorescent Leukocyte Angiography.

Yang, Yun Sik; Kim, Yoo Kang; Kim, Jae Duck

Original Article

Journal of the Korean Ophthalmological Society 1996;37(2):284-292.
Published online February 1, 1996.

A Study on Measurements of Retinal Blood Flow Using a Fluorescent Leukocyte Angiography.

Yun Sik Yang, Yoo Kang Kim, Jae Duck Kim

Department of Ophthalmology, College of Medicine, Wonkang University, Chunbuk, Korea.

형광백혁구 안저촬영술을 이용한 망막혈류 측정에 관한 연구

양연식(Y S Yang),김유강(Y K Kim),김재덜(J D Kim)

Abstract

This study was performed to develop a new method, fluorecent leukocyte angiography(FLAG), to measure retinal circulation. Fluorescein-stained leukocytes can be seen in all the retinal vessels during fluorescein angiography using Scanning Laser Ophthalmoscope(SLO FAG) and retinal blood flow can be measured by using leukocyte velocities in the retinal vessels. Methods were followings. Firstly, blood was withdrawn from vein, mixed with fluorescein(10%) and anticoagulant and then centrifuged. The yellowbrown coat layer containing fluorescin-stained leukocytes was selected and injected into the vein while performing SLO FAG. The image of FLAG displayed circulating hyperfluorescent leukocytes clearly in all retinal vessels, and allowed the measurement of leukocyte velocities. In conclusion, FLAG is a new method to measure blood flow in all the retinal vessels simultaneously and can be a useful tool for studying animal and human retinal circulation.

Key Words: Fluorescent leukocyte;Fluorescein angiography;Retinal blood flow;Scanning laser ophthalmoscope