Non-Electrolyte Permeability and Structural Changes of Rabbit Corneal Endothelium Stored in McCarey-Kaufman Medium.

Sohn, Kyung Sub; Kim, Jae Ho; Rhee, Sang Wook

Original Article

Journal of the Korean Ophthalmological Society 1977;18(1):135-142.
Published online January 1, 1977.

Non-Electrolyte Permeability and Structural Changes of Rabbit Corneal Endothelium Stored in McCarey-Kaufman Medium.

Kyung Sub Sohn, Jae Ho Kim, Sang Wook Rhee

Department of Ophthalmology, Catholic Meical College, Seoul, Korea.

McCarey - Kaufman 액에 보존한 토끼 각막 내피의 비전해질에 대한 투과성과 조직학적 변화

손경섭 , 김재호 , 이상욱 ( Kyung Sub Sohn , Jae Ho Kim , Sang Wook Lee )

Abstract

The permeability of rabbit corneal endothelium stored at 4 degrees C in M-K medium for period of 3, 5, 9 and 14 days to radioactive sucrose(molecular weight 360), inulin (molecular weight 5,200), and dextran (morecular weight 82,400) was measured. The corneal endothelium stored in M-K medium up to 14 days was examined with light and electron microscope. The endothelial permeability to non-electrolytes was decreased according to the molecular weight of these increased. The permeability of fresh cornea to suerose was about 4 times greater than to inulin and 50 times than to dextran, but in M-K medium stored groups the permeability to sucrose was about 2.5 times greater than to inulin and 36 times than to dextran. The permeability of M-K medium preserved cornea for 14 days was significantly different from that of fresh corneal endothelium. The light and electron microscopic findings of 3, 5 and 9 days stored corneal endothelium were almost normal except of early changes of mitochondria, but in the 14 days-stored groups there were marked degenerative change of cytoplasm and nucleus were noted.