Journal of the Korean Ophthalmological Society 1978;19(1):49-54.
Published online January 1, 1978.
Partial Penetrating Allografts of Rabbit Corneas Stored in M-K Medium.
Jae Ho Kim, Kyu Hyung Han, Duck Keun Yoon
1Department of Ophthalmology Catholic Medical College, Seoul, Korea.
2Ophthalmic Section, Choong-Chung-Buk-Do Medical Center, Chung-Joo City, Korea.
McCarey - Kaufman 액에 존재한 토끼 각막을 사용한 각막이식
김재호 , 한규형 ( Jae Ho Kim , Kyu Hyung Han )
Abstract
Each three rabbit corneas were stored in McCarey-Kaufman medium at 4 degrees C for 5, 9 and 14 days and used as donor materials in 6mm partial penetrating corneal allografts. These results were compared to that group of penetrating keratoplasties with corneas stored conventional moist chamber at 4 degrees C for 5 days. All the M-K media stored corneas for 5 and 9 days were in clear graft after keratoplasty during the observation period of 6 months but the corneas stored for 14 days were developed eventvally irreversilde opaque after grafts. At 6 months after keratoplasties allthe rabbits eyes were enucleated and sectioned for histopathologic study of endothelia with the light and electron microscopes. Endothelia of the corneas stored for 5 and 9 days showed rarely endothelial degenerative changes. But the corneas stored up to 14 days showed significant changes including of disappearanee of most endothelial membranes and cytoplasms in light microscopies and swelling of nucleus, dispersed chromatin clump, multiple small and large vacuoles in cytoplasm and occasional autolysis of cytoplasm were observed in electron microscopies.


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