A New Model of Extraocular Muscle Fibrosis by Thermal Cauterization in the Rats.

Choi, Min Gyu; Moon, Nam Ju

doi:2018.59.5.478

J Korean Ophthalmol Soc > Volume 59(5); 2018 > Article

Original Article

Journal of the Korean Ophthalmological Society 2018;59(5):478-483.
DOI: https://doi.org/10.3341/jkos.2018.59.5.478 Published online May 15, 2018.

A New Model of Extraocular Muscle Fibrosis by Thermal Cauterization in the Rats.

Min Gyu Choi, Nam Ju Moon

Department of Ophthalmology, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, Korea. njmoon@cau.ac.kr

열소작술을 이용한 흰쥐 외안근 섬유화 모델

최민규⋅문남주

중앙대학교 의과대학 중앙대학교병원 안과학교실

Correspondence: Nam Ju Moon,
Email: njmoon@cau.ac.kr

Received: 14 December 2017 • Revised: 16 February 2018 • Accepted: 23 April 2018

Abstract

PURPOSE
To evaluate the effect of cauterization on extraocular muscle (EOM) fibrosis in rats, and to develop a novel EOM fibrosis model. METHODS: Twenty-four eyes of 12 Sprague Dawley rats were assigned randomly to two groups. We exposed the superior rectus muscle (SRM) and performed thermal injury 2 mm behind the insertion site of the SRM using a cautery device in the experimental group. The thermal injuries were performed twice for 1 second, for a total of 2 seconds. In the control group, the same procedures except the thermal injury were performed. Two weeks after surgery, all eyes were enucleated and stained with hematoxylin and eosin (H&E) and Masson's trichrome (MT). RESULTS: Staining with H&E and MT showed that thermal injury significantly increased inflammation and fibrosis in the experimental group (p=0.002, p < 0.001, respectively). CONCLUSIONS: Thermal injury using cauterization effectively induced fibrosis of EOM in the rat model. This simple model was effective in inducing fibrosis of SRM and will be useful for studying postoperative fibrosis after strabismus surgery.

Key Words: Cauterization;Extraocular muscle;Fibrosis;Rat;Thermal injury