| Effect of Amniotic Membrane Extract on Cultured Human Nasal Mucosa Fibroblasts. |
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Seok Jin Hwang, Keun Hae Kim |
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Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. kimkh@cu.ac.kr |
| 배양된 사람 코점막 섬유아세포에 대한 양막추출물의 효과 |
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황석진⋅김근해 |
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대구가톨릭대학교 의과대학 안과학교실 |
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Received: 3 July 2015 • Revised: 7 August 2015 • Accepted: 25 September 2015 |
| Abstract |
PURPOSE
To investigate the effects of amniotic membrane extract (AME) on the survival of cultured human nasal mucosa fibroblasts. METHODS: Primary cultured human nasal mucosa fibroblasts were exposed to 0, 10, 20, or 30 microg/mL AME for 3 days. The survival of the human nasal mucosa fibroblasts was measured using the MTT assay and apoptosis was evaluated with flow cytometry using annexin-V/propidium iodide double staining. RESULTS: AME decreased significantly in fibroblast proliferation after exposure to 10 microg/mL (p = 0.000), and caused significant apoptosis of the fibroblasts after exposure to 10 microg/mL (p = 0.024). CONCLUSIONS: AME decreased fibroblast proliferation in vitro at least through induction of apoptosis. Therefore, adjuvant use of AME during endonasal dacryocystorhinostomy may improve clinical outcomes. |
| Key Words:
Amniotic membrane extract;Apoptosis;Nasal mucosa fibroblast |
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