J Korean Ophthalmol Soc > Volume 56(12); 2015 > Article
Journal of the Korean Ophthalmological Society 2015;56(12):1939-1944.
DOI: https://doi.org/10.3341/jkos.2015.56.12.1939    Published online December 15, 2015.
Effect of Amniotic Membrane Extract on Cultured Human Nasal Mucosa Fibroblasts.
Seok Jin Hwang, Keun Hae Kim
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. kimkh@cu.ac.kr
배양된 사람 코점막 섬유아세포에 대한 양막추출물의 효과
대구가톨릭대학교 의과대학 안과학교실
Received: 3 July 2015   • Revised: 7 August 2015   • Accepted: 25 September 2015
To investigate the effects of amniotic membrane extract (AME) on the survival of cultured human nasal mucosa fibroblasts. METHODS: Primary cultured human nasal mucosa fibroblasts were exposed to 0, 10, 20, or 30 microg/mL AME for 3 days. The survival of the human nasal mucosa fibroblasts was measured using the MTT assay and apoptosis was evaluated with flow cytometry using annexin-V/propidium iodide double staining. RESULTS: AME decreased significantly in fibroblast proliferation after exposure to 10 microg/mL (p = 0.000), and caused significant apoptosis of the fibroblasts after exposure to 10 microg/mL (p = 0.024). CONCLUSIONS: AME decreased fibroblast proliferation in vitro at least through induction of apoptosis. Therefore, adjuvant use of AME during endonasal dacryocystorhinostomy may improve clinical outcomes.
Key Words: Amniotic membrane extract;Apoptosis;Nasal mucosa fibroblast

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