J Korean Ophthalmol Soc > Volume 56(12); 2015 > Article
Journal of the Korean Ophthalmological Society 2015;56(12):1945-1952.
DOI: https://doi.org/10.3341/jkos.2015.56.12.1945    Published online December 15, 2015.
Long-Term Effect of Preservative-Free Sodium Hyaluronate Eye Drop on Human Corneal Epithelial Cell.
Jong Soo Lee, Jae Sung Park, Ho Yun Kim
1Department of Ophthalmology, Pusan National University Hospital, Busan, Korea. jongsool@pusan.ac.kr
2Department of Ophthalmology, Pusan National University School of Medicine, Busan, Korea.
3Lee Eye Clinic, Busan, Korea.
무보존제 히알루론산나트륨 점안이 각막상피세포에 미치는 장기적 영향
부산대학교병원 안과학교실1, 부산대학교 의학전문대학원 안과학교실2, 부산 이안과의원3
Received: 8 May 2015   • Revised: 22 June 2015   • Accepted: 16 October 2015
To investigate the biological effects of preservative-free artificial tear drops on cultured human corneal epithelial cells in vitro. METHODS: The effects of the preservative-free artificial tear drops (Kynex(R) 0.1%, Kynex II(R) 0.18% [Alcon, Seoul, Korea] and Hyaluni eye drops(R) 0.15%, 0.3% [Taejun, Seoul, Korea]) on the human corneal epithelial cells were evaluated. An methyl thiazolyl tetrazolium (MTT)-based colorimetric assay was performed to assess the cellular metabolic activity and a lactate dehydrogenase (LDH) leakage assay was used to determine cellular toxicity. The eye drop ingredients were analyzed for electrolyte composition, pH, and osmolarity. We performed a scratch assay and cellular morphology test using electronic microscopy. RESULTS: The metabolic activity of corneal epithelial cells was higher than controls at 24 hours after exposure and then decreased at 48 and 72 hours after exposure (p < 0.05). The LDH titers of the 4 eye drops were higher compared with controls (p < 0.05). Sodium hyaluronate 0.18% contained lower concentrations of Na+ or Cl- and showed lower osmolarity values compared with the other eye drops. The cellular migration based on the scratch assay was more delayed and cellular damage such as loss of microvilli, rough endothelial reticulum (RER), and mitochondria dilatation was greater than controls based on electron microscopy. CONCLUSIONS: Long-term exposure to preservative-free sodium hyaluronate eye drops may induce decreased metabolic activity and cellular damage. Thus, preservative-free artificial tears should be used carefully to prevent cellular toxicity.
Key Words: Artificial tear;Cornea;Epithelium;Hyaluronate;Keratocyte

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