| Expression of Nestin on Endothelial Cells and Pericytes During Retinal Vascular Development in Mouse. |
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Jin Soo Kim, Sung Wook Park, In Young Hwang, Yong Woo Kim, Jin Hyoung Kim, Jeong Hun Kim |
1Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea. steph25@snu.ac.kr
2Fight against Angiogenesis-Related Blindness Laboratory, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea.
3Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea.
4Department of Medicine, Seoul National University College of Medicine, Seoul, Korea. |
| 생쥐의 망막혈관 발생 과정에서 혈관내피세포 및 혈관주위세포의 네스틴 발현 분석 |
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김진수1⋅박성욱2,3⋅황인영4⋅김용우1⋅김진형2⋅김정훈1,2,3 |
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서울대학교 의과대학 안과학교실1, 서울대학교병원 의생명연구원 망막혈관실험실2, 서울대학교 의과대학 의과학과3, 서울대학교 의과대학 의학과4 |
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| Abstract |
PURPOSE
Nestin, a marker of neural stem cells, is expressed in Müller cells during retinal development. However, the role of nestin in retinal vascular development is not well established. Thus, we investigated the expression of nestin in developmental mouse retina and identified which retinal cells are related to the expression of nestin during the retinal vascular development. METHODS: Eyes were enucleated from C57BL/6 mice on postnatal day (P) 4, P8, P12, P16 and P26. Immunofluorescence was used to evaluate nestin expression in relation to endothelial cells (isolectin B4), pericytes (neural/glial antigen 2) and astrocytes (glial fibrillary acidic protein). RESULTS: Nestin was strongly expressed from the ganglion cell layer to retinoblast layer at P4. At P8, P12 and P16, the expression of nestin was observed from the upper border of the ganglion cell layer, and vertically penetrating to outer nuclear layer. At P26, the expression of nestin was decreased and confined to the ganglion cell layer and inner nuclear layer. Interestingly, there was strong vascular shape expression of nestin at all stages. The superficial, deep and intermediate vascular plexus was completely merged with nestin expression at P4, P8, P12 and P16. In addition, the nestin expression merged with pericytes but not with astrocytes. CONCLUSIONS: Nestin was expressed in endothelial cells and pericytes during retinal vascular development in the retina. These results suggest that nestin could play an important role in developmental angiogenesis via interplay with endothelial cells and pericytes. |
| Key Words:
Endothelial cell;Mouse retina;Nestin;Pericyte;Retinal vascular development |
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