| Comparison of Surface Roughness and Bacterial Adhesion between Cosmetic Contact Lenses and Conventional Contact Lenses. |
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Yong Woo Ji, Soon Ho Hong, Dong Yong Chung, Eung Kweon Kim, Hyung Keun Lee |
1Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. shadik@yuhs.ac
2Division of Microbiology, Department of Laboratory Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.
3Morphology Lab., Yonsei Biomedical Research Institute, Seoul, Korea.
4Institute of Corneal Dystrophy Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. |
| 미세표면분석 기법을 이용한 미용 콘택트렌즈와 일반 콘택트렌즈의 표면거칠기와 세균부착성 비교 |
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지용우1⋅홍순호2⋅정동룡3⋅김응권1,4⋅이형근1,4 |
Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine1, Seoul, Korea
Division of Microbiology, Department of Laboratory Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine2, Seoul, Korea
Morphology Lab., Yonsei Biomedical Research Institute3, Seoul, Korea
Institute of Corneal Dystrophy Research, Department of Ophthalmology, Yonsei University College of Medicine4, Seoul, Korea |
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| Abstract |
PURPOSE
To determine the surface roughness of cosmetic and conventional contact lenses (CLs) and their susceptibility to bacterial adhesion. METHODS: Concave surface roughness of cosmetic and conventional hydrogel (Etafilcon A) CLs was measured by atomic force microscopy (AFM) and scanning electron microscopy (SEM). In particular, the surface of the color tinted area of cosmetic CLs was measured. CLs were immersed into a bacterial solution of Pseudomonas aeruginosa for 1, 12, or 24 hours and culture of P. aeruginosa that had adhered to the CLs was performed. RESULTS: Concave surface roughness of cosmetic CLs significantly increased compared with conventional CLs by AFM (p < 0.05). Bacterial colony formation of P. aeruginosa adhering to cosmetic CLs within one hour significantly increased compared with conventional CLs (p = 0.047). Adhesions of P. aeruginosa to CLs within one hour was found to correlate significantly with the surface roughness of CL (r > 0.9, p < 0.05). By SEM, P. aeruginosa had adhered to the color-tinted area more than to the non-color-tinted area of cosmetic CLs. CONCLUSIONS: Surface of cosmetic CLs was significantly rougher and initial adhesion of bacteria was higher to cosmetic CLs than to conventional CLs. In particular, an increased number of bacteria was found to be adhered to the color-tinted area of cosmetic CLs. Initial bacterial adhesion is important because it is the first stage of bacterial attachment process to any surface. After then, the adherent bacteria can progress to form a biofilm. Increased surface roughness of CLs contributes to opportunities for the CL to come into contact with bacteria, and thus, initial bacterial adhesion increases. In this study, it is clear that cosmetic CLs are more vulnerable to bacterial adhesion. To avoid serious complications, such as bacterial keratitis, the manufacturing process for smoothing and treating the surface in order to inhibit bacterial adhesion should be developed in the future. |
| Key Words:
Atomic force microscope;Bacterial adhesion;Cosmetic contact lens;Pseudomonas aeruginosa;Surface roughness |
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