J Korean Ophthalmol Soc > Volume 55(8); 2014 > Article
Journal of the Korean Ophthalmological Society 2014;55(8):1195-1201.
DOI: https://doi.org/10.3341/jkos.2014.55.8.1195    Published online August 15, 2014.
Expression of TonEBP by Hypertonic and Hyperosmolar Stress in RGC-5 Cells.
Jong Eun Woo, Min Young Kwon, Su Wol Chung, Je Moon Woo
1Department of Ophthalmology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea. limbus68@naver.com
2School of Biological Sciences, College of Natural Science, University of Ulsan, Ulsan, Korea.
망막신경절세포에서 고장성 및 고오스몰 스트레스에 의한 긴장성 반응유도 결합단백질의 발현
우종은1⋅권민영2⋅정수월2⋅우제문1
Department of Ophthalmology, Ulsan University Hospital, University of Ulsan College of Medicine1, Ulsan, Korea
School of Biological Sciences, College of Natural Science, University of Ulsan2, Ulsan, Korea
Abstract
PURPOSE
In order to determine whether the Tonicity responsive enhancer binding protein (TonEBP) is expressed by hypertonic and hyperosmolar stress, TonEBP expression was investigated in the retinal ganglion cell (RGC) line, RGC-5 cells. METHODS: After RGC-5 cells were cultured by Staurosporine, TonEBP expression was measured with Western immunoblotting analysis and real-time reverse transcription-polymerase chain reaction in 50 mM NaCl, 100 mM mannitol, 50 mM glucose, or 100 mM glucose at 3, 6, 12, and 24 hours after exposure to each environment. RESULTS: In this study, the protein expression of TonEBP was determined to be statistically significantly checked in 50 mM NaCl after 3, and 6 hours, in 100 mM mannitol after 6 hours, and in 100 mM glucose after 3, and 6 hours. TonEBP messenger Ribonucleic acid (mRNA) expression was determined to be statistically significantly checked in 50 mM NaCl after 3 hours, in 100 mM mannitol after 3, and 24 hours, and in 50 mM glucose after 3, and 24 hours. CONCLUSIONS: These results suggested that TonEBP was expressed by hypertonic and hyperosmolar stress at the protein and mRNA levels. Further studies are nedded to determine the role of TonEBP and the mechanism of expression and regulation of TonEBP.
Key Words: Hyperosmolar stress;Hypertonic stress;Retinal ganglion cell death;TonEBP expression
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