Flow Cytometric Analysis of the Effects of Resveratrol on the Survival of Human Tennon's Capsule Fibroblasts.

Lee, See Eun; Kim, Keun Hae; Kim, Jae Woo

doi:2015.56.8.1268

J Korean Ophthalmol Soc > Volume 56(8); 2015 > Article

Original Article

Journal of the Korean Ophthalmological Society 2015;56(8):1268-1273.
DOI: https://doi.org/10.3341/jkos.2015.56.8.1268 Published online August 15, 2015.

Flow Cytometric Analysis of the Effects of Resveratrol on the Survival of Human Tennon's Capsule Fibroblasts.

See Eun Lee, Keun Hae Kim, Jae Woo Kim

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr

유세포분석을 이용한 레즈브라트롤이 인체 테논낭 섬유아세포의 생존에 미치는 영향에 대한 연구

이시은⋅김근해⋅김재우

대구가톨릭대학교 의과대학 안과학교실

Received: 12 February 2015 • Revised: 30 March 2015 • Accepted: 19 June 2015

Abstract

PURPOSE
Resveratrol exerts cytoprotective or cytotoxic effects according to cell type. This study was performed to evaluate the effects of resveratrol on the survival of cultured human Tenon's capsule fibroblasts (HTFBs). METHODS: Primarily cultured HTFBs were exposed to 0, 10, or 100 microM resveratrol for 3 days. Cellular survival was assessed using the MTT assay and degree of apoptosis was analyzed with flow cytometry using annexin-V/propidium iodide double staining. RESULTS: Resveratrol decreased the survival of HTFBs after exposure to 10 microM (p = 0.04). In flow cytometric analysis, 10 microM resveratrol did not affect the degree of apoptosis (p = 0.89), but 100 microM resveratrol increased the degree of apoptosis (p = 0.003). Both 10 and 100 microM resveratrol did not affect the degree of necrosis (p = 0.74, 0.33). CONCLUSIONS: Resveratrol decreased cellular survival of cultured HTFBs and induced apoptosis. Thus, resveratrol may exert antiproliferative effects on HTFBs.

Key Words: Apoptosis;Fibroblast;Flow cytometry;Resveratrol;Survival