| Effect of Cysteamine on Human Peripheral Blood Mononuclear Cells-Chemically Injured Keratocytes Reaction. |
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Young Bok Lee, Joon Young Hyon, Won Ryang Wee, Tae Young Chung, Eui Sang Chung, Ka Young Yi, Young Joo Shin |
1Department of Ophthalmology, Hallym University Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea. schinn@hanmail.net
2Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea.
3Department of Ophthalmology, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea.
4Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
5Rhee's Eye Hospital, Daejeon, Korea. |
| 말초혈액단핵구와 화학적으로 손상된 각막세포의 혼합반응에 대한 시스테아민의 효과 |
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이영복1⋅현준영2,3⋅위원량2⋅정태영4⋅정의상5⋅이가영1⋅신영주1 |
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한림대학교 의과대학 한림대학교 강남성심병원 안과학교실1, 서울대학교 의과대학 안과학교실2, 서울대학교 의과대학 분당서울대학교병원 안과학교실3, 성균관대학교 의과대학 삼성서울병원 안과학교실4, 대전 이안과병원5 |
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Received: 27 February 2015 • Revised: 30 May 2015 • Accepted: 14 August 2015 |
| Abstract |
PURPOSE
To investigate the effect of cysteamine on mixed peripheral blood mononuclear cells (PBMCs)-chemically injured keratocytes reaction (mixed lymphocyte-keratocyte reaction; MLKR). METHODS: PBMC stimulation assay was performed after keratocytes were chemically injured with 0.05 N NaOH for 60 seconds. MLKR was treated with various concentrations of cysteamine (0-10 mM). Intracellular reactive oxygen species (ROS) formation was measured using the oxidation-sensitive fluorescent probe, 2'7'-dichlorofluorescein diacetate (DCF-DA). Proliferation rate of PBMCs stimulated by NaOH-treated keratocytes and secretion profiles of matrix metalloprotease-9 (MMP-9), transforming growth factor-beta1 (TGF-beta1), interleukin-6 (IL-6), and macrophage migration inhibitory factor (MIF) were determined using the bromodeoxyuridine proliferation assay and enzyme-linked immunosorbent assay, respectively. RESULTS: Proliferation rate of PMBCs was suppressed by cysteamine in a dose-dependent manner (p = 0.019). Fluorescence of DCF-DA decreased depending on cysteamine concentration (p < 0.001). MMP-9, IL-6 and TGF-beta1 levels were suppressed by cysteamine in a dose-dependent manner (p < 0.05), whereas MIF levels increased with cysteamine concentration of 0.5-10 mM (p = 0.008). CONCLUSIONS: These study results indicate that cysteamine induced the ROS-mediated inhibition of inflammatory cytokine release and proliferation of PBMCs stimulated by chemically injured keratocytes. Thus, cysteamine can be used in the treatment of chemical corneal burns. |
| Key Words:
Cysteamine;Macrophage migration inhibitory factor;Mixed peripheral lymphocyte-keratocyte reaction;Reactive oxygen species;Transforming growth factor-beta1 |
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