Decorin and TGF-beta Expression after Partial Myotomy of the Extraocular Muscle in Rat.

Lim, Young Jin; Kim, Gyu Nam; Lee, Na Eun; Kim, Sung Jae; Yoo, Ji Myong

doi:2013.54.1.143

J Korean Ophthalmol Soc > Volume 54(1); 2013 > Article

Original Article

Journal of the Korean Ophthalmological Society 2013;54(1):143-148.
DOI: https://doi.org/10.3341/jkos.2013.54.1.143 Published online January 15, 2013.

Decorin and TGF-beta Expression after Partial Myotomy of the Extraocular Muscle in Rat.

Young Jin Lim, Gyu Nam Kim, Na Eun Lee, Sung Jae Kim, Ji Myong Yoo

¹Department of Ophthalmology, Gyeongsang National University School of Medicine, Jinju, Korea. YJM@gnu.ac.kr
²Institute of Health Science, Gyeongsang National University School of Medicine, Jinju, Korea.

쥐의 외안근 부분 절개술 후 데코린과 종양성장인자-베타의 발현

임영진¹⋅김규남¹⋅이나은¹⋅김성재¹⋅유지명^1,2

Department of Ophthalmology, Gyeongsang National University School of Medicine¹, Jinju, Korea
Institute of Health Science, Gyeongsang National University School of Medicine², Jinju, Korea

Abstract

PURPOSE
To report the expression of decorin and TGF-beta in partial myotomy of the extraocular muscle in rats. METHODS: Partial myotomy of the superior rectus muscle was performed on the right eye of 10 Sprague-Dawley rats followed by exposure of the left superior rectus muscle and a simple suture of the conjunctiva. The bilateral superior rectus muscle was obtained from all rats at 2 weeks postoperatively. The tissues were observed under light microscopy with hematoxylin-eosin, Masson's trichrome staining and immunohistochemistry. RESULTS: Histological examinations of the surgical area at 2 weeks after postoperatively showed irregularly concentrated fibrosis on light microscopy with hematoxylin-eosin and Masson's trichrome staining of the experimental eyes. Immnohistochemistry showed that expression of decorin was in the same location as TGF-beta in the experimental group. CONCLUSIONS: The expression of decorin was found in the healing process after partial myotomy of the extraocular muscle in rats. Immunohistochemistry showed that expression of decorin was in the same location as with TGF-beta.

Key Words: Decorin;Extraocular muscle;Fibrosis;Tumor growth factor