| A Study of the Pathway of Nitric Oxide Production by Nitroglycerin in Trabecular Meshwork Cells. |
|
Suk Hyun Yoon, Jae Woo Kim |
|
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 섬유주세포에서 니트로글리세린에 의한 일산화질소의 생성경로에 관한 연구 |
|
윤숙현⋅김재우 |
|
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea |
|
|
| Abstract |
PURPOSE
To investigate the effects of nitroglycerin on the production of nitric oxide and its related pathway in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 10 nM nitroglycerin using 1% serum-containing media for 30 minutes. The production of nitric oxide was assessed with the Griess assay and expressions of eNOS mRNA was assessed with RT-PCR. Additionally, the cells were exposed to wortmanin and Akt1/2 kinase inhibitor to investigate the mechanism related to the production of nitric oxide. RESULTS: Nitroglycerin increased the production of nitric oxide (p < 0.05) accompanied with increased expression of eNOS mRNA. The increased production of nitric oxide and eNOS mRNA was inhibited by wortmanin and Akt1/2 kinase inhibitor. CONCLUSIONS: Low-dose nitroglycerin increased the production of nitric oxide accompanied by increased eNOS activity. Nitroglycerin drives eNOS activation via the phosphatidylinositol 3-kinase/protein kinase B pathway. |
| Key Words:
eNOS;Nitroglycerin;Nitric oxide;Trabecular meshwork cells |
PDF Links
Full text via DOI
Download Citation
