| Effect of Preservative-free Artificial Eye Drop on Human Corneal Epithelial Cell in vitro. |
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Su Jin Kim, Eun Hee Kim, Ji Eun Lee, Jong Soo Lee |
1Department of Ophthalmology, Pusan National University School of Medicine, Medical Research Institute, Pusan National University Hospital, Busan, Korea. jongsool@pusan.ac.kr
2Department of Ophthalmology, St. Mary's Hospital, Busan, Korea. |
| 무방부제 인공누액이 인체각막상피세포에 미치는 영향 |
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김수진1ㆍ김은희2ㆍ이지은1ㆍ이종수1 |
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Department of Ophthalmology, Pusan National University School of Medicine, Medical Research Institute, Pusan National University Hospital1, Busan, Korea Department of Ophthalmology, St. Mary’s Hospital2, Busan, Korea |
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| Abstract |
PURPOSE
To investigate the biologic effects of preservative-free artificial tear drops on cultured human corneal epithelial cells in vitro. METHODS: Efficacies of the preservative-free artificial tear drops-Kynex(R), Hyalein Mini 0.3%(R), and Refresh Plus(R)-were evaluated using the MTT assay. Cell damage was determined using the lactate dehydrogenase (LDH) assay. Cellular proliferation was determined using a migration and wound-healing assay. The ingredients of the drugs were analyzed. Apoptotic response was evaluated with flow cytometric analysis. RESULTS: Metabolic activity of the corneal epithelial cells showed similar activity to that of the control. Cellular migration and proliferation also were not significantly different between the preservative-free artificial tear drop groups and the control. The LDH titers tended to increase for up to 24 hours after exposure to the preservative-free artificial tear drops, but there was no significant difference in LDH titers between the control groups and the artificial tear drop-treated groups. Apoptosis and necrosis were observed using flow cytometry at 24 hours in all groups. The electrolyte levels, pHs and osmolarities of the three drugs were not significantly different. CONCLUSIONS: The clinically available preservative-free artificial tear drops Kynex(R), Hyalein Mini 0.3%(R), and Refresh Plus(R) had no significant toxic effects on corneal epithelial cells and thus can be used safely. |
| Key Words:
Corneal epithelial cell toxicity;LDH;MTT;Preservative-free artificial tear |
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