The Study of Characteristics of Acellular Porcine Cornea Using Freezing-Thawing-Centrifugation. |
Seok Hyun Lee, Yeoun Sook Chun, Jae Chan Kim |
Department of Ophthalmology, Chungang University Yongsan Hospital, Seoul, Korea. jck50ey@kornet.net |
동결-해동-원심분리를 이용한 무세포 돼지각막의 특성 연구 |
이석현ㆍ전연숙ㆍ김재찬 |
Department of Ophthalmology, Chungang University Yongsan Hospital, Seoul, Korea |
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Abstract |
PURPOSE To develop a new decellularization technique of porcine cornea using freezing-thawing-centrifugation (FTC) and to examine the characteristics of acellular porcine cornea (APC) for xenograft material. METHODS: Two-hundred micrometer thickness porcine corneas were decellularized with DNase/RNase, followed by 3 freezing-thawing-centrifugations (FTC, group 1), lyophilized FTC-APC (group 2), and chemical enzyme treated APC (CE-APC, group 3). Histologic evaluation to examine cells and collagen matrix, comparison of transparency, and cultivation to determine the viability of stromal cells was performed in fresh porcine cornea and 3 experimental groups. RESULTS: Decellularization occurred successfully in all experimental groups. Decellularization was confirmed by H&E staining and cultivation. Transparency of group 1 was similar to the normal porcine cornea but transparency of group 2 and group 3 was decreased. Collagen fibers of CE-APC (group 3) were not as well arrayed as FTC-APC (group 2). CONCLUSIONS: Acellularity of porcine cornea was successfully achieved by the FTC method with preservation of the cornea stroma. Novel decellularized porcine cornea can be considered as xenogeneic material for corneal transplantation. |
Key Words:
Centrifuge;Decellularization;Porcine cornea;Xenograft |
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