J Korean Ophthalmol Soc > Volume 52(1); 2011 > Article
Journal of the Korean Ophthalmological Society 2011;52(1):86-92.
DOI: https://doi.org/10.3341/jkos.2011.52.1.86    Published online January 15, 2011.
The Study of Characteristics of Acellular Porcine Cornea Using Freezing-Thawing-Centrifugation.
Seok Hyun Lee, Yeoun Sook Chun, Jae Chan Kim
Department of Ophthalmology, Chungang University Yongsan Hospital, Seoul, Korea. jck50ey@kornet.net
동결-해동-원심분리를 이용한 무세포 돼지각막의 특성 연구
이석현ㆍ전연숙ㆍ김재찬
Department of Ophthalmology, Chungang University Yongsan Hospital, Seoul, Korea
Abstract
PURPOSE
To develop a new decellularization technique of porcine cornea using freezing-thawing-centrifugation (FTC) and to examine the characteristics of acellular porcine cornea (APC) for xenograft material. METHODS: Two-hundred micrometer thickness porcine corneas were decellularized with DNase/RNase, followed by 3 freezing-thawing-centrifugations (FTC, group 1), lyophilized FTC-APC (group 2), and chemical enzyme treated APC (CE-APC, group 3). Histologic evaluation to examine cells and collagen matrix, comparison of transparency, and cultivation to determine the viability of stromal cells was performed in fresh porcine cornea and 3 experimental groups. RESULTS: Decellularization occurred successfully in all experimental groups. Decellularization was confirmed by H&E staining and cultivation. Transparency of group 1 was similar to the normal porcine cornea but transparency of group 2 and group 3 was decreased. Collagen fibers of CE-APC (group 3) were not as well arrayed as FTC-APC (group 2). CONCLUSIONS: Acellularity of porcine cornea was successfully achieved by the FTC method with preservation of the cornea stroma. Novel decellularized porcine cornea can be considered as xenogeneic material for corneal transplantation.
Key Words: Centrifuge;Decellularization;Porcine cornea;Xenograft


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