Analysis of CCL5 Concentration in Tears of Dry Eye Patients.

Choi, Won; Lee, Seung Hyun; Yoon, Kyung Chul

doi:2011.52.6.658

J Korean Ophthalmol Soc > Volume 52(6); 2011 > Article

Original Article

Journal of the Korean Ophthalmological Society 2011;52(6):658-664.
DOI: https://doi.org/10.3341/jkos.2011.52.6.658 Published online June 15, 2011.

Analysis of CCL5 Concentration in Tears of Dry Eye Patients.

Won Choi, Seung Hyun Lee, Kyung Chul Yoon

Department of Ophthalmology, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea. kcyoon@chonnam.ac.kr

건성안 환자의 눈물 내 케모카인 리간드 5의 농도 분석

최 원⋅이승현⋅윤경철

Department of Ophthalmology, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea

Abstract

PURPOSE
To investigate the expression of CCL5/RANTES (regulated upon activation, normal T cell expressed and secreted) in the tears of dry eye patients. METHODS: Forty patients with dry eye (15 Sjogren's and 25 non-Sjogren's syndrome patients) and ten control subjects were recruited for the present study. The concentration of RANTES in tears was measured using an enzyme-linked immunosorbent assay. The correlations between RANTES level, tear film and ocular surface parameters, including tear film break-up time, basal tear secretion, tear clearance rate, corneal sensation, keratoepitheliopathy, and conjunctival goblet cell density, were analyzed in patients with dry eye syndrome. RESULTS: The concentrations of RANTES were 435.46 +/- 104.45 pg/ml in Sjogren's syndrome patients, 257.42 +/- 46.72 pg/ml in non-Sjogren's syndrome patients, and 97.53 +/- 29.15 pg/ml in the control patients (p < 0.01). The levels correlated significantly with basal tear secretion, tear clearance rate, keratoepitheliopathy, and goblet cell density (p < 0.05). CONCLUSIONS: CCL5/RANTES level increases in the tears of dry eye patients and correlates with various tear film and ocular surface parameters.

Key Words: CCL5/RANTES;Dry eye;Ocular surface;Sjogren's syndrome;Tear film