| Effects of Calf Serum on Human Corneal Epithelial Cells in Vitro. |
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Jong Soo Lee, Eun Hee Kim, Su Jin Kim |
1Department of Ophthalmology, Pusan National University School of Medicine, Medical Research Institute, Pusan National University Hospital, Busan, Korea. jongsool@pusan.ac.kr
2Department of Ophthalmology, Busan St. Mary's Medical Center, Busan, Korea. |
| 송아지 혈청 농도가 인체의 각막상피세포에 미치는 영향 |
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이종수1⋅김은희2⋅김수진1 |
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Department of Ophthalmology, Pusan National University School of Medicine, Medical Research Institute, Pusan National University Hospital1, Busan, Korea Department of Ophthalmology, Busan St. Mary’s Medical Center2, Busan, Korea |
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| Abstract |
PURPOSE
To investigate the biologic effects of topical calf serum on corneal epithelial cells in vitro. METHODS: The effects of calf serum on the corneal epithelial cells were evaluated using the MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, and the concentration of IL-1alpha, TGF-beta1 and MMP-9 in the cells was measured. Cell damage was determined using lactate dehydrogenase (LDH), and cellular morphologies were examined by transmission electromicroscopy. RESULTS: Metabolic activity of the corneal epithelial cells decreased at higher concentrations and longer exposure durations. IL-1alpha, TGF-beta1 and MMP-9 titers were lower in calf serum-treated cells than in the control. LDH and cellular damage to the corneal epithelial cells, such as chromatin margination and cytoplasmic organelle swelling, were prominent in cells treated with 30% calf serum. CONCLUSIONS: Cellular metabolic activity was higher and cellular toxicity was lower in cells treated with 10% calf serum compared to those treated with the 20% and 30% concentrations. Furthermore, inflammatory cytokines were sufficiently inhibited in cells treated with the 10% solution. These results indicate that 10% calf serum could be used clinically. |
| Key Words:
Calf serum;Corneal epithelial cell toxicity;LDH;MTT |
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