Autophagy of Human Tenon's Capsule Fibroblasts Induced by Mitomycin-C. |
Channy Park, Raekil Park, Seong Kwang Ryu, Dong Wook Lee |
1Department of Audiology, Nambu University, Gwangju, Korea. 2VestibuloCochlear Research Center & Department of Microbiology, Wonkwang University, Iksan, Korea. 3Department of Ophthalmology, Chonbuk National University Medical School, Jeonju, Korea. ldw@jbnu.ac.kr |
마이토마이신씨에 의해 유도된 인간 테논낭 섬유아세포의 자가포식현상 |
박찬희1,2⋅박래길1,2⋅유성광3⋅이동욱3 |
Department of Audiology, Nambu University1, Gwangju, Korea VestibuloCochlear Research Center & Department of Microbiology, Wonkwang University2, Iksan, Korea Department of Ophthalmology, Chonbuk National University Medical School3, Jeonju, Korea |
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Abstract |
PURPOSE The present study investigated whether an autophagic process is involved in the apoptotic death of human tenon's capsule fibroblasts (HTCFs) caused by mitomycin-C. METHODS: An autophagic phenotype was tested using fluorescence microscopy and flow cytometry with specific biological staining dyes including monodansylcadaverine and acridine orange and microtubule-associated protein 1 light chain 3 (LC3). RESULTS: Treatment with mitomycin-C (0.4 mg/ml) increased the acidic vesicular organelles of tenon's capsule fibroblasts in a time dependent manner. Mitomycin-C induced both LC3-II cleavage and beclin-1 expression. 3-MA, a pharmacological inhibitor of autophagy, inhibited the mitomycin-C induced increase of acidic vesicular organelleS. CONCLUSIONS: Autophagy was induced with 0.4 mg/ml mitomycin-C in tenon's capsule fibroblasts. And, autophagic mechanisms may be involved in the early stage of apoptosis of fibroblasts. |
Key Words:
Autophagy;Fibroblast;Mitomycin-C |
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