| Comparison of Vitreolytic Effect in Rabbit Eyes: Plasmin, Hyaluronidase, and Their Mixtures. |
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Moo Sang Kim, Sang Woong Moon, Eung Suk Kim, Seung Young Yu, Hyung Woo Kwak |
1Department of Ophthalmology, KyungHee University Hospital, Seoul, Korea. hwkwak@khmc.or.kr
2Department of Ophthalmology, Inje University College of Medicine, Seoul Paik Hospital, Seoul, Korea.
3Department of Ophthalmology, Chung-Ang University College of Medicine, Seoul, Korea. |
| 토끼 눈에서 플라스민과 히알루론산분해효소의 약리적 유리체융해능력 비교 |
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김무상1ㆍ문상웅2ㆍ김응석3ㆍ유승영1ㆍ곽형우1 |
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Department of Ophthalmology, KyungHee University Hospital1, Seoul, Korea / Department of Ophthalmology, Inje University College of Medicine, Seoul Paik Hospital2, Seoul, Korea / Department of Ophthalmology, Chung-Ang University College of Medicine3, Seoul, Korea |
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| Abstract |
PURPOSE
The aim of the present study was to quantify and compare the vitreolytic effect of plasmin, hyaluronidase, and a combination of the two. METHODS: Thirty-six rabbits were randomized into 3 groups: (A) twelve rabbits had an intravitreal injection of plasmin 1 U with hyaluronidase 10 U/0.1 mL into the right eye, (B) twelve rabbits had an injection of plasmin alone (1 U/0.1 mL), and (C) twelve rabbits had an injection of hyaluronidase alone (10 U/0.1 mL). The left eye of each rabbit was used as control, which was injected with 0.1 mL phosphate buffered saline (PBS). The eyes were enucleated 1 hour and 24 hours after injection. The volume of fluid-type vitreous and gel-type vitreous was measured with a micropipette using the melting point as the difference. Statistical analysis was performed and light microscopy was used to assess potential damage to the retinal tissue. RESULTS: The volume of remaining gel-type vitreous was measured as 52.5%, 60.3%, 59.2%, and 76.5% after 1 hour enucleation and as 44.6%, 56.7%, 56.1%, and 74.7%, after 24 hours enucleation in group A, B, C, and control group, respectively. Group A, B, and C showed statistically significant differences against the control group. Group A (plasmin with hyaluronidase) showed less remaining gel-type vitreous volume than a single injection of plasmin or hyaluronidase alone. CONCLUSIONS: Intravitreal injection of plasmin with hyaluronidase showed more vitreolytic effect than a single injection of plasmin or hyaluronidase alone. The enzyme may be useful in liquefying the vitreous, and may be a useful biochemical adjunct to vitrectomy. |
| Key Words:
Hyaluronidase;Intravitreal injection;Pharmacologic vitreolysis;Tissue plasminogen activator |
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