J Korean Ophthalmol Soc > Volume 51(5); 2010 > Article
Journal of the Korean Ophthalmological Society 2010;51(5):733-739.
DOI: https://doi.org/10.3341/jkos.2010.51.5.733    Published online May 15, 2010.
Analysis of the Effective Fraction of Sun Ginseng Extract in Selenite Induced Cataract Rat Model.
Sang Mok Lee, Jung Moon Sun, Jin Ho Jeong, Mee Kum Kim, Won Ryang Wee, Jeong Hill Park, Jin Hak Lee
1Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea. jjhlee@plaza.snu.ac.kr
2Department of Ophthalmology, The Armed Forces Capital Hospital, Seongnam, Korea.
3Department of Ophthalmology, Soonchunhyang University College of Medicine, Cheonan, Korea.
4Department of Ophthalmology, Incheon Medical Center, Incheon, Korea.
5Artificial Eye Center of Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.
6Seoul National University College of Pharmacy, Seoul, Korea.
7Department of Ophthalmology, Seoul National University Bundang Hospital, Seongnam, Korea.
셀레나이트로 유도되는 백내장에서 선삼 성분에 따른 백내장 억제효과의 비교 분석
이상목1,2ㆍ정문선3ㆍ정진호4ㆍ김미금1,5ㆍ위원량1,5ㆍ박정일6ㆍ이진학1,7
Department of Ophthalmology, Seoul National University College of Medicine1, Seoul, Korea Department of Ophthalmology, The Armed Forces Capital Hospital2, Seongnam, Korea Department of Ophthalmology, Soonchunhyang University College of Medicine3, Cheonan, Korea Department of Ophthalmology, Incheon Medical Center4, Incheon, Korea Artificial Eye Center of Clinical Research Institute, Seoul National University Hospital5, Seoul, Korea Seoul National University College of Pharmacy6, Seoul, Korea Department of Ophthalmology, Seoul National University Bundang Hospital7, Seongnam, Korea
Abstract
PURPOSE
To compare the protective effects of saponin and non-saponin Sun-ginseng extract fractions in a selenite-induced rat cataract model. METHODS: A total of 101 Sprague-Dawley rat pups were divided into four groups by treatment: Sun-ginseng, saponin fraction, non-saponin fraction, and control. For induction of cataracts, sodium selenite 15 nmol/g was injected subcutaneously in 13 day-old rat pups. Sun-ginseng extract 100 microgram/g (Group I, Ginseng Science, Seoul, Korea), saponin fraction 100 microgram/g (Group II), non-saponin fraction 100 microgram/g (Group III), and phosphate buffered saline (Control group) were injected intraperitoneally every two days for a total of seven injections. The rats were sacrified and their lenses were dissected and photographed at day 7 and 14, and the cataracts were graded according to the ratio of the cataract area to the total lens area. The blind method was used for the evaluation of the cataract area. RESULTS: At day 14, cataract formation rates (CFR) were 33.3% in group I, 76.4% in group II, 41.2% in group III, and 77.7% in the control group. The mean cataract area (MCA) was 13.4+/-20.8% in group I, 14.4+/-11.7% in group II, 5.7+/-7.7% in group III, and 15.8+/-12.1% in the control group. Group III showed statistically significant results compared with those of control group (CFR p=0.001, MCA p=0.001). We observed significantly lower incidence and smaller mean cataract area in Group I and Group III at day 7 compared with the control group (Group I, CFR p=0.018; Group III, CFR p=0.032, MCA p=0.005). CONCLUSIONS: The protective effects of Sun-ginseng extract are caused by the components in the non-saponin fraction, not by those in the saponin fraction, in a selenite-induced cataract rat model.
Key Words: Cataract;Rat;Saponin;Selenite;Sun ginseng


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