| Effects of Damaged Human Corneal Epithelial Cells on Differentiation of Human Mesenchymal Stem Cell. |
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Mi Sun Shin, Hyun Sook Hong, Young Sook Son, Jae Chan Kim |
1Department of Ophthalmology Chung-ang University College of Medicine, Seoul Korea. jck50ey@kornet.net
2Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, Seoul, Korea. |
| 손상 받은 각막 상피세포가 중배엽 줄기세포의 분화에 미치는 영향 |
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신미선1,홍현숙2,손영숙2,김재찬1 |
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Department of Ophthalmology Chung-ang University College of Medicine1, Seoul Korea Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences2, Seoul, Korea |
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Correspondence:
Jae Chan Kim, M.D.1 |
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| Abstract |
PURPOSE
To identify the effects of microenvironmental changes caused by human corneal epithelial damages to characteristics or differentiation of human mesenchymal stem cells (hMSCs). METHODS: Artificial corneal damage was induced onto a cultured monolayer of human corneal epithelial cells. hMSCs were then co-cultured with damaged human corneal epithelial cells (dIHCE). Morphological changes in the co-cultured hMSCs were observed. To elucidate the differentiation of hMSCs into corneal keratocytes or epithelial cells, the expressions of alpha-smooth muscle actin, keratin-3/-12, and E-cadherin were confirmed by immunofluorescence. RESULTS: hMSCs co-cultured with dIHCE showed enhanced adherence in the neighborhood of dIHCE and morphological change into dendritic shapes at 6 days post-seeding. Although the expression of alpha-smooth muscle actin, known as hMSCs marker, significantly decreased at the dIHCE-contacted site of hMSCs; there were no expressional changes on keratin-3/-12 and E-cadherin, the markers of corneal epithelial cells. Interestingly, positive expression of corneal epithelial marker keratin-3/-12 was observed in dIHCE co-cultured hMSCs. hMSCs co-cultured with normal human corneal epithelial cells (nIHCE) were unable to attach, and showed no change in the expression of alpha-smooth muscle actin. CONCLUSIONS: It is proposed that dIHCE causes a morphological change in hMSCs, and decreased expression of alpha-smooth muscle actin. These results suggest that dIHCE can affect a change in the characteristics and differentiation of hMSCs. |
| Key Words:
Mesenchymal stem cell;Cornea epithelial cell;Coculture;alpha-smooth muscle actin |
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