| Evaluation of Cysticidal Effects of Contact Lens Disinfectant on Acanthamoeba Isolates. |
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Ji Eun Lee, Jae Sung Park, Jong Soo Lee, Hak Sun Yoo |
1Department of Ophthalmology, Pusan National UniversityCollege of Medicine, Pusan, Korea. jongsool@pusan.ac.kr
2Department of Parasitology, Pusan National UniversityCollege of Medicine, Pusan, Korea. |
| 콘택트렌즈 소독액의 아메바 포낭에 대한 살충능력 평가 |
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이지은1,박재성1,이종수1,유학선2 |
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Department of Ophthalmology, Pusan National UniversityCollege of Medicine1, Pusan, Korea Department of Parasitology, Pusan National UniversityCollege of Medicine2, Pusan, Korea |
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Correspondence:
Ji Eun Lee, M.D.1 |
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| Abstract |
PURPOSE
To evaluate the cysticidal effect of 5 kinds of commercially available contact lens disinfectants against 2 clinical isolates of Acanthamoeba. METHODS: Five kinds of commercially available contact lens disinfectants were soaked with cysts of Acanthamoeba ludgdunesis and castellanii at the concentration of 10(3), 10(4), and 10(5) cells/ml for 1 and 4 or 6 hours. Cysts which were not excysted in 7 days after treatment were recognized to be killed. Morphologic changes were evaluated by electron microscopic observation. RESULTS: Contact lens disinfectants which contain myristamidopropyl dimethylamine (MAPD) showed the best cysticidal effect. These disinfectants demonstrated a cysticidal effect on both Acanthamoeba species of all concentrations in 6-hour treatment. Contact lens disinfectants which contain polyhexamethylene biguanide (PHMB) did not demonstrate cysticidal effect, except for Acanthamoeba castellanii at the concentration of 10(3) cells/ml, in either 4- or 6-hour treatment. Separation of plasma membrane from endocyst and damage of organelles were prominent in cases showing a cysticidal effect. CONCLUSIONS: Contact lens disinfectant which contains MAPD may be helpful in preventing the Acanthamoeba keratitis. A higher concentration of PHMB is required to be effective in preventing Acanthamoeba keratitis. |
| Key Words:
Acanthamoeba;Amoebicidal effect;Contact lens disinfectants;Cyst |
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