| Predictability of Donor Lamellar Graft Thickness and Diameter Using a Microkeratome in Porcine Eyes. |
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Moo Sang Kim, Seung Chan Lee, Seung Jun Lee, Kyung Hyun Jin |
1Department of Ophthalmology, KyungHee University College of Medicine, Seoul, Korea. khjinmd@khmc.or.kr
2Department of Ophthalmology, Kangwon National University, College of Medocine, Kangwon, Korea. |
| 미세각막절개도를 이용하여 얻은 돼지눈 표층이식편의 두께 및 지름 예측성 |
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김무상1,이승찬2,이승준2,진경현1 |
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Department of Ophthalmology, KyungHee University College of Medicine1, Seoul, Korea Department of Ophthalmology, Kangwon National University, College of Medocine2, Kangwon, Korea |
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Correspondence:
Moo Sang Kim, M.D.1 |
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| Abstract |
PURPOSE
To evaluate the thickness and diameter of a lamellar graft with microkeratome and to determine its usefulness as a donor lamellar graft for microkeratome-assisted lamellar keratoplasty. METHODS: Thirty-six porcine eyes were randomly assigned to three groups according to the microkeratome (Automated Corneal Shaper(R)) depth plate: group 1 (#300 micrometer plate, 13 eyes), group 2 (#400 micrometer plate, 12 eyes), and group 3 (#450 micrometer plate, 11 eyes). Free corneal buttons were made with the corneal shaper after a uniform suction duration of 10 seconds. Central corneal thicknesses were measured with an automated ultrasound pachymeter prior to the procedure. After the lamellar graft was made, the remaining corneal thickness was measured with the same method. The difference between the two values was hypothesized to be the lamellar graft thickness. In addition, the diameter of lamellar graft was measured by calipers. RESULTS: The mean lamellar graft thicknesses in groups 1, 2, and 3 were 218.2+/-26.6 micrometer (72.7%), 312.3+/-26.7 micrometer (78.1%) and 345.7+/-36.7 micrometer (76.8%), respectively. There were no significant differences between the three groups according to the results brought by the microkeratome depth plates (p=0.239). The mean lamellar graft diameters in groups 1, 2, and 3 were 8.49+/-0.28 mm, 8.43+/-0.26 mm and 8.58+/-0.28 mm, respectively. There were no significant differences between the three groups (p=0.368). No relationship was found between lamellar graft thickness and diameter (r=-0.009, p=0.480). CONCLUSIONS: These results suggest that in forming donor lamellar grafts with microkeratome plates, thicker resection depth is needed because the actual lamellar graft were shown to be thinner than the target values. However, lamellar grafting is thought to be unsuitable for lamellar keratoplasty because it results in considerable thickness variability. |
| Key Words:
Automated Corneal Shaper;Lamellar graft;Lamellar keratoplasty;Microkeratome |
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