| Proteomic Analysis of the Vitreous with Proliferative Diabetic Retinopathy. |
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Sung Jin Lee, Sung Ho Lee, Song Hee Park, Sung Chul Lee, Oh Woong Kwon |
1Department of Ophthalmology, Soonchunhyang University College of Medicine, Seoul, Korea.
2Department of Proteomics, R&D Center, EyeGene Inc., Seoul, Korea.
3Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. owkwon0301@yumc.yonsei.ac.kr |
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이성진1,이성호2,박성희1,이성철3,권오웅3 |
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Department of Ophthalmology, Soonchunhyang University College of Medicine1. Seoul, Korea Department of Proteomics, R&D Center, EyeGene Inc.2, Seoul, Korea Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine3, Seoul, Korea |
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Correspondence:
Sung Jin Lee, M.D.1 |
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| Abstract |
PURPOSE
This study analyzed protein alterations between the normal vitreous and the vitreous with proliferative diabetic retinopathy by proteomics to find the proteins which may elicit diabetic retinopathy. METHODS: Two-dimensional electrophoresis was used to make the protein map. Image analysis between the spots on each gels by a proteomics based approach were used to reveal vitreous protein alterations which may elicit proliferative diabetic retinopathy. MALDI-TOF/ESI-TOF mass spectrometry also was used to identify altered protein spots on the gel. RESULTS: Of the 110 different spots on each gels, 36 different proteins were identified and among them 23 proteins were altered in the vitreous with proliferative diabetic retinopathy compared with normal vitreous. Nineteen proteins including alpha-1-antitrypsin, Ig G and A, and complement C3 and C4 were increased in the vitreous with proliferative diabetic retinopathy and 4 proteins includng pigment epithelium derived factor were decreased compared to the normal vitreous. CONCLUSIONS: The authors found that pigment epithelium derived factor may be the key protein that induces the neovascularization in the vitreous with proliferative diabetic retinopathy. Increased levels of Ig G and A and C3 and C4 is thought to be related to the autoimmune inflammation in early diabetic microangiopathy. Furthermore, proteins such as alpha-1-antitrypsin may contribute to protective functions of the ischemic retinal cells. |
| Key Words:
Diabetic retinopathy;Electrophoresis;MALDI-TOF;Protein;Vitreous |
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