| Effect of Nitric Oxide on the Trabecular Meshwork Cell-mediated Contraction of Collagen Gels. |
|
Jae Woo Kim, Woo Seok Chang, Su Yoon Lee |
|
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 일산화질소가 섬유주세포에 의한 교원질 겔의 수축에 미치는 영향 |
|
김재우.장우석.이수윤 |
|
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea |
|
Correspondence:
Jae Woo Kim, M.D., Ph.D. |
|
|
| Abstract |
PURPOSE
To investigate the effect of nitric oxide (NO) on the contraction of cultured human trabecular meshwork cells (HTMCs). METHODS: After embedding them into collagen gels, primarily cultured HTMCs were exposed to NO donors, such as sodium nitroprusside (SNP) or S-Nitroso-N-acetylpenicillamine (SNAP), for 1 week at various concentrations, and the contraction of the collagen gels was measured. Cellular survival and NO production were measured with MTT assay and Griess assay, respectively. RESULTS: Though SNP and SNAP did not significantly affect cellular survival, they markedly enhanced NO production. Both sodium nitroprusside and SNAP inhibited the contraction of collagen gels by about 10% in dose and time-dependent manners (p<0.05). CONCLUSIONS: NO donors inhibited the contraction of collagen gels in vitro. Thus, NO donors may relax trabecular meshwork and enhance trabecular outflow. |
| Key Words:
Collagen gel;Contraction;Nitric oxide;Trabecular meshwork |
|
PDF Links
Full text via DOI
Download Citation
