| Effect of Hydrogen Peroxide-induced Oxidative Stress on the Senescence of Trabecular Meshwork Cells. |
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Jae Woo Kim, Sin Hoo Kim, Jae Hyung Lee |
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Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Koera. jwkim@cu.ac.kr |
| 과산화수소에 의한 산화스트레스가 섬유주세포의 노화에 미치는 영향 |
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김재우ㆍ김신후ㆍ이재형 |
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Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Koera |
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| Abstract |
PURPOSE
To investigate the effects of oxidative stress on the senescence of trabecular meshwork (TM) cells and the effect of L-ascorbic acid (LAA) against oxidative stress-induced senescence. METHODS: Primary cultured human TM cells were exposed to 0.05 or 0.1 mM hydrogen peroxide for 30 minutes and incubated for 1 week with or without co-exposure of LAA. Cellular survival, nitrite production, and senescence were assessed with MTT, Griess, and SA-beta-gal assays, respectively. RESULTS: Hydrogen peroxide decreased cellular survival and NO production accompanied increased cellular senescence. LAA did not prevent hydrogen peroxide-induced senescence. CONCLUSIONS: Oxidative stress-induced senescence of TM cells may be related to the dysfunction of trabecular meshwork in glaucoma. |
| Key Words:
Ascorbic acid;Hydrogen peroxide;Oxidative stress;Senescence;Trabecular meshwork cells |
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