| Inhibitory Effect of Rapamycin on Corneal Neovascularization induced by Angiogenin in Rabbits. |
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Young Sam Kwon, Soo Ik Chang, Dong Ku Kang, Jae Chan Kim |
1Department of Ophthalmology, Chung-Ang University, College of Medicine, Seoul, Korea.
2Department of Biochemistry, Chungbuk National University, College of Natural Science, Chungju, Korea. jck50ey@kornet.net |
| 엔지오제닌에 의한 가토 각막 혈관신생에 대한 라파마이신의 억제 효과 |
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권영삼1,장수익2,강동구2,김재찬1 |
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Department of Ophthalmology, Chung-Ang University, College of Medicine1, Seoul, Korea Department of Biochemistry, Chungbuk National University, College of Natural Science2, Chungju, Korea |
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Correspondence:
Young Sam Kwon, Ph.D.,1 |
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| Abstract |
PURPOSE
The purpose of this study was to determine whether immunosuppressant, rapamycin could inhibit corneal angiogenesis induced by angiogenin and to evalutate its role by micropocket assay. METHODS: The rabbit's eye was implanted intrastromally into the superior cornea with pellet for the control group, pellet containing of angiogenin for the angiogenin group, and pellet containing of angiogenin and rapamycin for the angiogenin+rapamycin group. Biomicrographically, corneal angiogenesis was evaluated for 14 days after pellet implantation, based on the number and the length of new vessels. The neovascularized cornea also was examined histologically. RESULTS: We could observe that the angiogenin inducing corneal angiogenesis was inhibited by rapamycin. The score of neovascularization was significantly decreased in the angiogenin+rapamycin group than in the angiogenin group at 3, 7 and 10 days after pellet implantation (p<0.05). Histologically, the cornea of angiogenin+rapamycin group also showed much less new vessels than that of angiogenin group, in which inflammatory cells and edema was observed. CONCLUSIONS: Rapamycin appears to inhibit angiogenin induced angiogenesis in a rabbit corneal micropocket assay and may have therapeutic potential as an antiangiogenic agent. |
| Key Words:
Angiogenin;Neovascularization;Rapamycin |
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