Axoplasmic Transport of Herpes Simplex Virus Co-Cultured with Ciliary Nerve.

Kang, Suk Won; Ko, Myung Kyoo

Original Article

Journal of the Korean Ophthalmological Society 2005;46(9):1575-1581.
Published online September 30, 2005.

Axoplasmic Transport of Herpes Simplex Virus Co-Cultured with Ciliary Nerve.

Suk Won Kang, Myung Kyoo Ko

Department of Ophthalmology, Hanyang University College of Medicine, Seoul, Korea. fovea@hanyang.ac.kr

섬모체 신경조직과 동시 배양된 단순포진바이러스의 축삭이동

강석원,고명규

Department of Ophthalmology, Hanyang University College of Medicine¹, Seoul, Korea

Correspondence: Suk-Won Kang, M.D.

Abstract

PURPOSE
To investigate the replication of HSV within cultured cell and axonal transport of HSV within the axon of the ciliary nerve following the injection of HSV into a cultured ciliary nerve. METHODS: The explant of the ciliary nerve was cultured with a medium containing nerve growth factor for 30 days when the suspension of HSV-1 (Kos strain) was introduced into the culture dish to co-culture with the ciliary nerve. The ciliary nerve was examined with transmission electron microscopy 30 days after culture and 6 days after co-culture with HSV. RESULTS: The ultrastructure of the explant of the ciliary nerve co-cultured with HSV showed that the viral capsid acquired a viral envelope and viral core, and a capsid and inclusion body within the nucleus. The enveloped virus was scattered within the vesicles of the cytoplasm. The virus-like particles were identified at the axonal fibers. CONCLUSIONS: The co-culture of the explant of the ciliary nerve and HSV showed the replicative process of the HSV within the cultured cell. The virus-like particles within the axon showed the evidence axonal transport of the virus under culture conditions.

Key Words: Axonal transport;Ciliary nerve;Culture;Herpes simplex virus;Ultrastructure