| Effect of Oxidative Stress on the Expression of Gelatinases in Cultured RPE. |
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Young Chul Park, Gwang Ju Choi |
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Department of Ophthalmology, Chosun University College of Medicine, Gwangju, Korea. gjchoi@chosun.ac.kr |
| 배양망막색소상피세포에서 젤라틴아제의 발현에 대한 산화스트레스의 영향 |
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박영철,최광주 |
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Department of Ophthalmology, Chosun University College of Medicine, Gwangju, Korea |
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Correspondence:
Young-Chul Park, M.D. |
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| Abstract |
PURPOSE
To evaluate the expression pattern of gelatinases in the human retinal pigment epithelial cell line ARPE-19 in response to oxidative stress. METHODS: ARPE-19 cells were exposed to paraquat for 72 hours. The cells were assayed for the mRNA expression of gelatinase A and gelatinase B by reverse transcriptase polymerase chain reaction (RT-PCR). The conditioned media were assayed for the production of gelatinases protein by Western blotting. Also, the inhibitory effect of the antioxidants against oxidative stress to the cells was evaluated. RESULTS: The gelatinase levels in the RPE cells increased under oxidative stress compaired with the control (p<0.05). Also, the levels decreased when the cells were incubated with the antioxidant N-acetylcysteine (NAC). CONCLUSIONS: Oxidative stress upregulates gelatinase expression in ARPE-19 cells. |
| Key Words:
Antioxidant;Matrix metalloproteinase;Oxidative stress;Retinal pigment epithelial cell |
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