| Gene Microarray Related with Apoptosis in Diabetic OLETF Keratocytes. |
|
Ji Eun Lee, Hae Song Kim, Jong Soo Lee |
1Department of Ophthalmology, College of Medicine, Pusan National University, Pusan, Korea. jongsool@pusan.ac.kr
2Mi-Rae Ai center Eye Clinic, Pusan, Korea. |
| 당뇨쥐의 각막실질세포에서 세포자멸사와 관련된 유전자 연구 |
|
이지은1,김해송2,이종수1 |
|
Department of Ophthalmology, College of Medicine, Pusan National University1, Pusan, Korea Mi-Rae Ai center Eye Clinic2, Pusan, Korea |
|
Correspondence:
Ji Eun Lee, M.D.1 |
|
|
| Abstract |
PURPOSE
This study was to identified differentiated genes concerned with apoptosis that are up-regulated or down-regulated in OLETF corneal keratocytes stimulated with interleukin-1alpha. METHODS: Otsuka Long Evans Tokushima Fatty (OLETF) corneal keratocytes were primarily cultured and treated with 20 ng/ml of IL-1a for 6 hours. Hybridization was carried out using Oligonucleotide microarray. A sample of normal keratocytes was labeled with Cy 3, and a diabetic keratocytes sample was labeled with Cy 5. RESULTS Diabetes showed 20 down-regulated genes and 24 up-regulated genes compared with normal keratocytes. Also, IL-1alpha-treated diabetic keratocytes expressed 31 down-regulated and 33 up-regulated genes. Compared with diabetic keratocytes, the newly expressed genes in OLETF treated with IL-1alpha were Bcl, Lam, Timp, Mmp, Socs, Bmp, Ccl, Lcn, C7, etc. CONCLUSIONS: There were some genes related with apoptosis that expressed differently between normal and diabetic keratocytes of OLETF. Also, IL-1alpha may stimulate differing effects of apoptosis on diabetic corneal keratocytes. Studies analyzing the apoptotic significance of these differences identified in diabetic keratocytes are needed. |
| Key Words:
Apoptosis;Diabetes mellitus;Keratocyte;Microarray;Rat |
|
PDF Links
Download Citation
