Journal of the Korean Ophthalmological Society 2007;48(1):125-134.
Published online January 31, 2007.
Effect of Amoebicidal Agents on the Human Corneal Keratocytes in Vitro.
Ji Eun Lee, Jong Soo Lee, Hee Young Choi, Hak Sun Yoo
1Department of Ophthalmology, Pusan National University, College of Medicine, Pusan. Korea. hychoi@pusan.ac.kr
2Department of Parasitology, Pusan National University, College of Medicine, Pusan. Korea.
각막기질세포에 대한 아칸트아메바각막염 치료제의 영향
이지은1,이종수1,최희영1,유학선2
Department of Ophthalmology, Pusan National University, College of Medicine,1 Pusan. Korea Department of Parasitology, Pusan National University, College of Medicine,2 Pusan. Korea
Correspondence:  Ji Eun Lee, M.D.1
Abstract
PURPOSE
To evaluate the effect of polyhexamethylene biguanide (PHMB) and chlorhexidine on Acanthamoeba cysts and cultured human keratocytes. METHODS: Each well of two-fold diluted PHMB and chlorhexidine were treated on the Acanthamoeba cyst suspension of 5 x 10(4) cysts/ml for 8, 24, and 48 hours to measure the minimal cysticidal concentration (MCC) of each disinfectant and was exposed to the human corneal keratocytes of 5 x 10(4) cells/ml for same hours to measure the survival rate of keratocytes. Inverted phase-contrast micrograph and electron microscopy for observing the morphologic changes were evaluated. RESULTS: MCC of PHMB was 9.42 microgram/ml, 5.62 microgram/ml, and 2.37 microgram/ml, and chlorhexidine was 24.32 microgram/ml, 10.02 microgram/ml, and 7.02 microgram/ml respecitvely in 8, 24, and 48 hours. The survival rate of keratocytes at MCC was 91.7%, 64.6%, and 49.7% in PHMB of which significant decreases were found at 24 and 48 hours, and 95.7%, 90.6%, and 78.1% in chlorhexidine of which significant decrease was only found at 48 hours. The higher the concentration of disinfectants, cysts and keratocytes demonstrated more damaged appearance. CONCLUSIONS: The amoebicidal efficacy of PHMB and chlorhexidine was similar. However, in consideration of toxic effect on keratocytes by disinfectants, chlorhexidine is suggested to be more clinically useful than PHMB.
Key Words: Acanthamoeba;Chlorhexidine;Human corneal keratocyte;Minimal cysticidal concentraion;Polyhexamethylene biguanide


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