Effect of Amoebicidal Agents on the Human Corneal Keratocytes in Vitro. |
Ji Eun Lee, Jong Soo Lee, Hee Young Choi, Hak Sun Yoo |
1Department of Ophthalmology, Pusan National University, College of Medicine, Pusan. Korea. hychoi@pusan.ac.kr 2Department of Parasitology, Pusan National University, College of Medicine, Pusan. Korea. |
각막기질세포에 대한 아칸트아메바각막염 치료제의 영향 |
이지은1,이종수1,최희영1,유학선2 |
Department of Ophthalmology, Pusan National University, College of Medicine,1 Pusan. Korea Department of Parasitology, Pusan National University, College of Medicine,2 Pusan. Korea |
Correspondence:
Ji Eun Lee, M.D.1 |
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Abstract |
PURPOSE To evaluate the effect of polyhexamethylene biguanide (PHMB) and chlorhexidine on Acanthamoeba cysts and cultured human keratocytes. METHODS: Each well of two-fold diluted PHMB and chlorhexidine were treated on the Acanthamoeba cyst suspension of 5 x 10(4) cysts/ml for 8, 24, and 48 hours to measure the minimal cysticidal concentration (MCC) of each disinfectant and was exposed to the human corneal keratocytes of 5 x 10(4) cells/ml for same hours to measure the survival rate of keratocytes. Inverted phase-contrast micrograph and electron microscopy for observing the morphologic changes were evaluated. RESULTS: MCC of PHMB was 9.42 microgram/ml, 5.62 microgram/ml, and 2.37 microgram/ml, and chlorhexidine was 24.32 microgram/ml, 10.02 microgram/ml, and 7.02 microgram/ml respecitvely in 8, 24, and 48 hours. The survival rate of keratocytes at MCC was 91.7%, 64.6%, and 49.7% in PHMB of which significant decreases were found at 24 and 48 hours, and 95.7%, 90.6%, and 78.1% in chlorhexidine of which significant decrease was only found at 48 hours. The higher the concentration of disinfectants, cysts and keratocytes demonstrated more damaged appearance. CONCLUSIONS: The amoebicidal efficacy of PHMB and chlorhexidine was similar. However, in consideration of toxic effect on keratocytes by disinfectants, chlorhexidine is suggested to be more clinically useful than PHMB. |
Key Words:
Acanthamoeba;Chlorhexidine;Human corneal keratocyte;Minimal cysticidal concentraion;Polyhexamethylene biguanide |
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