Journal of the Korean Ophthalmological Society 2004;45(8):1370-1379.
Published online August 1, 2004.
Histologic Analysis of the Myotoxic Change by Bupivacaine and Ricin mAb 35 on Extraocular Muscle in Rabbits.
Choul Yong Park, Sung Eun Park, Sei Yeul Oh
1Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. syoh@smc.samsung.co.kr
2Department of Ophthalmology, Eulji General Hospital, Eulji University, College of Medicine, Seoul, Korea.
부피바카인(Bupivacaine)과 리신 단클론항체 35 (Ricin mAb 35) 주사에 의한 가토 외안근 근독성 변화에 대한 조직학적 분석
박철용 ( Choul Yong Park ) , 박성은 ( Sung Eun Park ) , 오세열 ( Sei Yeul Oh )
Abstract
PURPOSE
To analyze the acute histologic change of extraocular muscles (EOM) induced by injection of bupivacaine or Ricin mAb 35. METHODS: The superior rectus and inferior rectus of white rabbits were injected with either bupivacaine (0.4 mg in 0.3ml) or Ricin mAb 35 (0.2 micro gram/kg in 0.3 ml). One, two, and four weeks after injection, the rectus muscles were harvested and the post-injection changes were histologically analyzed. RESULTS: Both the orbital and the global layers of EOM showed myotoxic changes induced by bupivacaine and Ricin mAb 35. However, the inflammation and destruction of myofiber by bupivacaine injection were localized to the injection site, whereas changes induced by Ricin mAb 35 were diffuse. Regenerating myofibers with a central nucleus were found at one week after myotoxin injection. Four weeks after injection, the acute changes induced by these two toxins were much recovered with prominent myofiber regeneration. Bupivacaine-induced myotoxic change was more prominent in the global layer in contrast to the more prominent damage in the orbital layer induced by Ricin mAb 35. CONCLUSIONS: We found that EOM have a superb ability to recover from the acute injury induced by bupivacaine or Ricin mAb 35 and that the two myotoxins cause unique damage including the predilection of muscle layers and the duration for which the damage persisted. Further investigation into the functional change during recovery from the myotoxin-induced injury of EOM is needed.
Key Words: Bupivacaine;EOM;Myotoxin;Ricin mAb 35


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