Journal of the Korean Ophthalmological Society 2004;45(9):1560-1566.
Published online September 1, 2004.
Ultrastructural Findings of Cultured Ciliary Nerve.
Yoon Goo Ahn, Byung Ro Lee, Myung Kyoo Ko
Department of Ophthalmology, Hanyang University College of Medicine, Seoul, Korea. fovea@hanyang.ac.kr
배양된 섬모체 신경조직의 미세구조
안윤구 ( Yoon Goo Ahn ) , 이병로 ( Byung Ro Lee ) , 고명규 ( Myung Kyoo Ko )
Abstract
PURPOSE
To investigate the cellular changes and cellular characteristics of myelinated nerve fiber, unmyelinated nerve fiber and Schwann cell under the culture conditions of the explant of the long ciliary nerve. METHODS: The ciliary nerve was obtained from the eyecup after removal of the cornea and intraocular contents following the division of the eyeball at the limbal zone. The explant of the ciliary nerve was cultured with DMEM medium containing nerve growth factor in an incubator with a 5% CO2 atmosphere. The cultured ciliary nerve was examined with phase contrast microscopy at 35 days after culture and stained in the culture dish with 1% methylene blue. The cultured ciliary nerve was also examined with transmission electron microscope. RESULTS: The phase contrast microscopy showed monolayered Schwann cells and cells with thin cytoplasmic processes. The ultrastructure showed myelinated nerve fiber, unmyelinated nerve fiber and Schwann cells entrapped in the nerve fiber. The axoplasm of the myelinated nerve fiber showed the degeneration of microorganelles whereas the unmyelinated nerve fiber showed well preserved microorganelles such as neurofilaments and mitochondria within the axoplasm. CONCLUSIONS: These findings suggest that the axoplasm of the unmyelinated nerve fiber under these culture conditions is relatively better preserved than that of the myelinated nerve fiber probably due to the absence of the myelin sheath. Further study will be required to investigate whether the myelin sheath of the ciliary nerve derived from peripheral neuropathy may affect the functional change of the anterior segment of the eye.
Key Words: Ciliary nerve;Culture;Myelin;Nerve fiber;Schwann cell;Ultrastructure


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