Expression of growth factor, extracellular matrix and antioxidant (N-acetylcysteine) effect in TGF beta1 treated rat lens system. |
Min Soo Park, Chung Sook Ahn, Young Sook Hong |
1Gong Eye Medical Center, Korea. meanpark@hanmail.net 2Department of Ophthalmology, Ewha Womans University College of Medicine, Korea. 3Department of Biochemistry, Ewha Womans University College of Medicine, Korea. |
TGF β1으로 처리한 흰쥐 수정체에서 성장인자, 세포외기질의 발현과 수정체에서 성장인자 발현과 항산화제에 대한 연구 |
박민수 ( Min Soo Park ) , 안정숙 ( Chung Sook Ahn ) , 홍영숙 ( Young Sook Hong ) |
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Abstract |
PURPOSE The objective of this study was to characterize transdifferentiated lens epithelial cells analyzed by reverse transcription-polymerase chain reaction (RT-PCR) for the expression of mRNAs encoding growth factors, growth factor receptors and pathologic extracellular matrix proteins and by Western blot analysis for the proteins encoded by these mRNAs. Moreover, after antioxidants treatment, such as Nacetyl cysteine (NAC), we observed the effect on changes in the expression of growth factors, growth factor receptors and extracellular matrix proteins. METHODS: TGF-beta treated rat lens cultured with medium 199 (Sigma Co. St. Louis, MO) was subject to RT-PCR and Western blot analysis to assess expression of mRNAs and proteins encoded by these mRNAs. RESULTS: The expression of mRNAs for TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta receptor, epidermal growth factor (EGF), epidermal growth factor receptor, fibroblast growth factor (FGF), fibroblast growth factor receptor and connective tissue growth factor (CTGF) were increased. The levels of type I collagen, fibronectin, and alpha-smooth muscle actin (SMA) mRNAs were also increased. However, the expression of growth factors, receptors, extracellular matrix were decreased by antioxidant, such as NAC. CONCLUSIONS: The enhanced expression of growth factors, growth factor receptors and extracellular matrix in present the molecular mechanism underlying pathogenesis of cataracts. And the suppression of growth factors and growth factor receptors with treatment of antioxidants, such as NAC, suggests the possibility of using drugs in the prevention or treatment of cataracts. |
Key Words:
Lens epithelial cell;mRNA;Growth factors;Antioxidants |
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