Effects of Taxol and Mitomycin C on Morphologic Alteration of Cultured Rabbit Subconjunctival Fibroblasts.

Baek, Yong Woo; Moon, Jung Il; Park, Chan Kee

Original Article

Journal of the Korean Ophthalmological Society 2003;44(1):202-207.
Published online January 1, 2003.

Effects of Taxol and Mitomycin C on Morphologic Alteration of Cultured Rabbit Subconjunctival Fibroblasts.

Yong Woo Baek, Jung Il Moon, Chan Kee Park

¹Department of Ophthalmology, St. Mary's Hospital, College of Medicine, The Catholic University of Korea. jimoon@catholic.ac.kr
²Department of Ophthalmology, Kang-Nam St. Mary's Hospital, College of Medicine, The Catholic University of Korea.

배양된 가토의 결막하 섬유모세포에 Taxol과 Mitomycin C가 미치는 형태학적인 변화

백용우 ( Yong Woo Baek ) , 문정일 ( Jung Il Moon ) , 박찬기 ( Chan Kee Park )

Abstract

PURPOSE
Scar tissue formation by cell proliferation, migration and contraction of fibroblasts may result in the failure of trabeculectomy. The author investigated effects of Mitomycin C (MMC) and Taxol on morphologic alteration of cultured rabbit subconjunctival fibroblasts. METHODS: Indirect immunofluorescence microscopy, with antibodies against tubulin, was used to examine the effect of Mitomycin C and Taxol on cytoplasmic microtubules in rabbit. Tenon's fibroblast cells and Acridine orange/Ethidium bromide dyes were used to determine cell viability and apoptosis at various concentrations of MMC and Taxol. RESULTS: Taxol treated cell showed an increased intensity of microtubule staining around the nuclear region and morphologic differences in overall cell shape, and MMC treated cells were noted more prominent apoptotic changes. CONCLUSIONS: Our result suggested that Taxol might be as potent inhibitor of fibroblast proliferation as Mitomycin C, and therefore, might be used for improving the success rate of trabeculectomy.

Key Words: Fibroblast;Microtubule;Mitomycin C;Taxol